A rapid densitometric method for the analysis of hyoscyamine and scopolamine in solanaceous plants and their transformed root cultures

被引：11

作者：

Berkov, S

Pavlov, A

机构：

[1] Bulgarian Acad Sci, Inst Bot, BU-1113 Sofia, Bulgaria

[2] Bulgarian Acad Sci, Inst Microbiol, Plovdiv 4002, Bulgaria

来源：

PHYTOCHEMICAL ANALYSIS | 2004年 / 15卷 / 03期

关键词：

gas chromatography; thin layer chromatography-densitometry; tropane alkaloids; hyoscyamine; scopolamine; hairy root cultures;

D O I：

10.1002/pca.756

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and convenient method for sample preparation and simultaneous densitometric quantification of hyoscyamine (1) and scopolamine (2) has been developed. The alkaloid profiles of plant samples, as well as the content of 1 and 2, determined by TLC-densitometry corresponded well with those obtained using a GC method. The proposed method is simple and sensitive and can be used for the routine assay of 1 and 2, and for screening for alkaloid-rich and biochemically-interesting transformed root cultures and intact solanaceous plants. Copyright (C) 2004 John Wiley Sons, Ltd.

引用

页码：141 / 145

页数：5

共 13 条

[1]

[Anonymous], ALKALOIDS

[2] A rapid quantitative method for the analysis of galanthamine and other amaryllidaceae alkaloids by capillary column gas chromatography [J].