Adaptation of lung antioxidants to cigarette smoking in humans

We investigated the effect of free radical scavengers, micronutrient antioxidants, on antioxidant enzyme activities in cigarette smokers. We measured the intracellular superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities and vitamin E and beta-carotene levels in the bronchoalveolar cells of 14 smokers before and after 6 weeks of supplementation with vitamins E and C and beta-carotene. Eight nonsmokers served as control subjects, CAT and GPs activities mere higher in BAL, cells from smokers compared with nonsmokers (20.5+/-2.3 vs 9.6+/-1.3 U/10(6) cells; p=0.027; 0.90+/-0.10 vs 0.46+/-0.12 U/10(6) cells; p=0.049, respectively), while there was no difference in SOD activity between the two groups. Likewise, vitamin E and beta-carotene concentrations were markedly higher in smokers' lung lavage cells (403.3+/-81.0 in smokers vs 16.6+/-5.3 ng/10(6) cells in nonsmokers, and 1.23+/-0.21 in smokers vs 0.15+/-0.04 ng/10(6) cells in nonsmokers, respectively), The serum levels of vitamin E and C and beta-carotene increased by 2.0-, 1.6-, and 8.9-fold in smokers after supplementation, which were similar to nonsmokers. Similarly, BAL cell vitamin E increased from 403.3+/-81.0 to 477.4+/-97.7 ng/10(6) cells and beta-carotene increased from 1.23+/-0.21 to 4.32+/-0.45 ng/10(6) cells (p<0.05). Despite increased concentrations of vitamins in serum as well as beta-carotene levels in BAL, cells, there was no significant down regulation in SOD, CAT, or GPx activities in the lung lavage cells. These data suggest that augmentation of micronutrient antioxidants in smokers and bbential regulation of these defenses.