Intracytoplasmic delivery of anionic proteins

被引：51

作者：

Dalkara, D ^{[1
]}

Zuber, G ^{[1
]}

Behr, JP ^{[1
]}

机构：

[1] Univ Louis Pasteur Strasbourg 1, Lab Chim Genet, CNRS, Fac Pharm, F-67401 Illkirch Graffenstaden, France

来源：

MOLECULAR THERAPY | 2004年 / 9卷 / 06期

关键词：

immunocytochemistry; antibody; cationic lipid; protein delivery; gene silencing;

D O I：

10.1016/j.ymthe.2004.03.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Protein delivery is emerging as an interesting alternative to gene delivery. We have used our experience with transfection to develop a technique for efficient delivery of anionic proteins such as antibodies into the cytoplasm of cells. As for DNA, when complexed with cationic lipids, large amounts of proteins are shown to enter adherent cells via ubiquitously expressed syndecans. However, protein surface area rather than electric charge ratio governs the delivery characteristics. Delivery of anti-beta-actin and anti-alpha-tubulin IgG's leads to fiber depolymerization. Intracellular delivery of an antibody could thus be regarded as another method for interfering with gene activity.

引用

页码：964 / 969

页数：6

共 18 条

[1] EFFICIENT GENE-TRANSFER INTO MAMMALIAN PRIMARY ENDOCRINE-CELLS WITH LIPOPOLYAMINE-COATED DNA [J].