MIZIP, a highly conserved, vertebrate specific melanin-concentrating hormone receptor 1 interacting zinc-finger protein

被引：16

作者：

Bächner, D ^{[1
]}

Kreienkamp, HJ ^{[1
]}

Richter, D ^{[1
]}

机构：

[1] Univ Hamburg, Inst Zellbiochem & Klin Neurobiol, D-20246 Hamburg, Germany

来源：

FEBS LETTERS | 2002年 / 526卷 / 1-3期

关键词：

yeast-two-hybrid; MYND-zinc finger; G protein-coupled receptor interacting protein; food-intake behavior;

D O I：

10.1016/S0014-5793(02)03115-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using the yeast-two-hybrid system a novel protein was identified from human brain that interacts with the C-terminus of melanin-concentrating hormone receptor 1 (MCH-R1). This protein, characterized by a Myeloid translocation protein 8, Nervy, DEAF1 proteins (MYND) zinc-finger domain, is termed MCH-R1-interacting zinc-finger protein, MIZIP. It is fully conserved in man, rat, mouse and highly conserved in Xenopus and zebrafish, but not detectable in invertebrates. MIZIP gene organization in human (six exons on chromosome 9q34.3) and mouse is highly conserved, yet in rodents an additional exon is generated giving rise to alternatively spliced mRNAs. MIZIP is expressed in brain, testis and stomach, where expression of MCH and MCH-R1 was previously reported. MIZIP interaction with MCH-R1 was verified by overlay and pull-down assays as well as by co-transfection experiments in human embryonic kidney-293 cells. MIZIP is cytoplasmically localized but gets recruted to the plasma membrane when cells are co-transfected with MCH-R1 supporting the notion that MIZIP is involved in the function of MCH-R1. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：124 / 128

页数：5

共 20 条

[1] Identification of melanin concentrating hormone (MCH) as the natural ligand for the orphan somatostatin-like receptor 1 (SLC-1) [J].

Bächner, D ;

Kreienkamp, HJ ;

Weise, C ;

Buck, F ;

Richter, D .

FEBS LETTERS, 1999, 457 (03) :522-524

[2] Neuropeptides and obesity [J].

Beck, B .

NUTRITION, 2000, 16 (10) :916-923

[3] Signaling at zero G: G-protein-independent functions for 7-TM receptors [J].

Brzostowski, JA ;

Kimmel, AR .

TRENDS IN BIOCHEMICAL SCIENCES, 2001, 26 (05) :291-297

[4] DEAF-1, a novel protein that binds an essential region in a Deformed response element [J].

Gross, CT ;

McGinnis, W .

EMBO JOURNAL, 1996, 15 (08) :1961-1970

[5] The melanin-concentrating hormone receptor couples to multiple G proteins to activate diverse intracellular signaling pathways [J].

Hawes, BE ;

Kil, E ;

Green, B ;

O'Neill, K ;

Fried, S ;

Graziano, MP .

ENDOCRINOLOGY, 2000, 141 (12) :4524-4532

[6] PRO-MELANIN CONCENTRATING HORMONE MESSENGER-RIBONUCLEIC-ACID AND PEPTIDES EXPRESSION IN PERIPHERAL-TISSUES OF THE RAT [J].

HERVIEU, G ;

NAHON, JL .

NEUROENDOCRINOLOGY, 1995, 61 (04) :348-364

[7] The distribution of the mRNA and protein products of the melanin-concentrating hormone (MCH) receptor gene, slc-1, in the central nervous system of the rat [J].

Hervieu, GJ ;

Cluderay, JE ;

Harrison, D ;

Meakin, J ;

Maycox, P ;

Nasir, S ;

Leslie, RA .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2000, 12 (04) :1194-1216

[8] Melanin-concentrating hormone receptor is a target of leptin action in the mouse brain [J].

Kokkotou, EG ;

Tritos, NA ;

Mastaitis, JW ;

Slieker, L ;

Maratos-Flier, E .

ENDOCRINOLOGY, 2001, 142 (02) :680-686

[9] Cloning of the rat brain cDNA encoding for the SLC-1 G protein-coupled receptor reveals the presence of an intron in the gene [J].

Lakaye, B ;

Minet, A ;

Zorzi, W ;

Grisar, T .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1998, 1401 (02) :216-220

[10]

Lerman MI, 2000, CANCER RES, V60, P6116

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