Control of intercalation is cell-autonomous in the notochord of Ciona intestinalis

被引:40
作者
Keys, DN [1 ]
Levine, M [1 ]
Harland, RM [1 ]
Wallingford, JB [1 ]
机构
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
关键词
Dishevelled; convergent extension; cell intercalation; morphogenesis; Ciona; Xenopus; planar cell polarity;
D O I
10.1006/dbio.2002.0656
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Dishevelled signaling plays a critical role in the control of cell intercalation during convergent extension in vertebrates. This study presents evidence that Dishevelled serves a similar function in the Ciona notochord. Embryos transgenic for mutant Dishevelled fail to elongate their tails, and notochord cells fail to intercalate, though notochord cell fates are unaffected. Analysis of mosaic transgenics revealed that the effects of mutant Dishevelled on notochord intercalation are cell-autonomous in Ciona, though such defects have nonautonomous effects in Xenopus. Furthermore, our data indicate that notochord cell intercalation in Ciona does not require the progressive signals which coordinate cell intercalation in the Xenopus notochord, highlighting an important difference in how mediolateral cell intercalation is controlled in the two animals. Finally, this study establishes the Ciona embryo as an effective in vivo system for the study of the molecular control of morphogenetic cell movements in chordates. (C) 2002 Elsevier Science (USA).
引用
收藏
页码:329 / 340
页数:12
相关论文
共 66 条
[1]   The domineering non-autonomy of frizzled and Van Gogh clones in the Drosophila wing is a consequence of a disruption in local signaling [J].
Adler, PN ;
Taylor, J ;
Charlton, J .
MECHANISMS OF DEVELOPMENT, 2000, 96 (02) :197-207
[2]   Differential recruitment of Dishevelled provides signaling specificity in the planar cell polarity and Wingless signaling pathways [J].
Axelrod, JD ;
Miller, JR ;
Shulman, JM ;
Moon, RT ;
Perrimon, N .
GENES & DEVELOPMENT, 1998, 12 (16) :2610-2622
[3]   Dishevelled: at the crossroads of divergent intracellular signaling pathways [J].
Boutros, M ;
Mlodzik, M .
MECHANISMS OF DEVELOPMENT, 1999, 83 (1-2) :27-37
[4]   Dishevelled activates JNK and discriminates between JNK pathways in planar polarity and wingless signaling [J].
Boutros, M ;
Paricio, N ;
Strutt, DI ;
Mlodzik, M .
CELL, 1998, 94 (01) :109-118
[5]  
CLONEY RICHARD A., 1964, ACTA EMBRYOL MORPHOL EXP, V7, P111
[6]  
Conklin E.G., 1905, J ACAD NATL SCI PHIL, V13, P1, DOI DOI 10.5962/BHL.TITLE.4801
[7]  
Corbo JC, 1997, DEVELOPMENT, V124, P2335
[8]  
Corbo JC, 1997, DEVELOPMENT, V124, P589
[9]   The ascidian as a model organism in developmental and evolutionary biology [J].
Corbo, JC ;
Di Gregorio, A ;
Levine, M .
CELL, 2001, 106 (05) :535-538
[10]   Switching on the notochord [J].
Cunliffe, VT ;
Ingham, PW .
GENES & DEVELOPMENT, 1999, 13 (13) :1643-1646