Developmentally restricted actin-regulatory molecules control morphogenetic cell movements in the zebrafish gastrula

被引:37
作者
Daggett, DF [1 ]
Boyd, CA
Gautier, P
Bryson-Richardson, RJ
Thisse, C
Thisse, B
Amacher, SL
Currie, PD
机构
[1] Western Gen Hosp, MRC, Comparat & Dev Genet Sect, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
[2] Univ Strasbourg 1, Inst Natl Sante & Rech Med, Inst Genet & Biol Mol Mol & Cellulaire, CNRS, F-67404 Illkirch Graffenstaden, France
[3] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
关键词
D O I
10.1016/j.cub.2004.08.024
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although our understanding of the regulation of cellular actin and its control during the development of invertebrates is increasing [1-9], the question as to how such actin dynamics are regulated differentially across the vertebrate embryo to effect its relatively complex morphogenetic cell movements remains poorly understood. Intercellular signaling that provides spatial and temporal cues to modulate the subcellular localization and activity of actin regulatory molecules represents one important mechanism [10-13]. Here we explore whether the localized gene expression of specific actin regulatory molecules represents another developmental mechanism. We have identified a cap1 homolog and a novel guanine nucleotide exchange factor (GEF), quattro (quo), that share a restricted gene expression domain in the anterior mesendoderm of the zebrafish gastrula. Each gene is required for specific cellular behaviors during the anterior migration of this tissue; furthermore, capl regulates cortical actin distribution specifically in these cells. Finally, although capl and quo are autonomously required for the normal behaviors of these cells, they are also nonautonomously required for convergence and extension movements of posterior tissues. Our results provide direct evidence for the deployment of developmentally restricted actin-regulatory molecules in the control of morphogenetic cell movements during vertebrate development.
引用
收藏
页码:1632 / 1638
页数:7
相关论文
共 41 条
[1]   Has2 is required upstream of Rac1 to govern dorsal migration of lateral cells during zebrafish gastrulation [J].
Bakkers, J ;
Kramer, C ;
Pothof, J ;
Quaedvlieg, NEM ;
Spaink, HP ;
Hammerschmidt, M .
DEVELOPMENT, 2004, 131 (03) :525-537
[2]   Spatial control of the actin cytoskeleton in Drosophila epithelial cells [J].
Baum, B ;
Perrimon, N .
NATURE CELL BIOLOGY, 2001, 3 (10) :883-890
[3]   Act up controls actin polymerization to alter cell shape and restrict hedgehog signaling in the Drosophila eye disc [J].
Benlali, A ;
Draskovic, I ;
Hazelett, DJ ;
Treisman, JE .
CELL, 2000, 101 (03) :271-281
[4]   Rho and Rac take center stage [J].
Burridge, K ;
Wennerberg, K .
CELL, 2004, 116 (02) :167-179
[5]   The multidomain protein Trio binds the LAR transmembrane tyrosine phosphatase, contains a protein kinase domain, and has separate rac-specific and rho-specific guanine nucleotide exchange factor domains [J].
Debant, A ;
SerraPages, C ;
Seipel, K ;
OBrien, S ;
Tang, M ;
Park, SH ;
Streuli, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (11) :5466-5471
[6]   Rho GTPases in cell biology [J].
Etienne-Manneville, S ;
Hall, A .
NATURE, 2002, 420 (6916) :629-635
[7]  
Freeman NL, 2000, CELL MOTIL CYTOSKEL, V45, P106, DOI 10.1002/(SICI)1097-0169(200002)45:2<106::AID-CM3>3.0.CO
[8]  
2-3
[9]   Shaping the zebrafish notochord [J].
Glickman, NS ;
Kimmel, CB ;
Jones, MA ;
Adams, RJ .
DEVELOPMENT, 2003, 130 (05) :873-887
[10]   Wnt/Frizzled activation of Rho regulates vertebrate gastrulation and requires a novel formin homology protein Daam1 [J].
Habas, R ;
Kato, Y ;
He, X .
CELL, 2001, 107 (07) :843-854