Bioinformatics construction of the human cell surfaceome

被引：99

作者：

da Cunha, J. P. C. ^{[2
]}

Galante, P. A. F. ^{[2
]}

de Souza, J. E. ^{[2
]}

de Souza, R. F. ^{[2
]}

Carvalho, P. M. ^{[2
]}

Ohara, D. T. ^{[2
]}

Moura, R. P. ^{[2
]}

Oba-Shinja, S. M. ^{[3
]}

Marie, S. K. N. ^{[3
]}

Silva, W. A., Jr. ^{[4
]}

Perez, R. O. ^{[5
]}

Stransky, B. ^{[6
]}

Pieprzyk, M. ^{[7
]}

Moore, J. ^{[7
]}

Caballero, O. ^{[1
]}

Gama-Rodrigues, J. ^{[5
]}

Habr-Gama, A. ^{[5
]}

Kuo, W. P. ^{[8
]}

Simpson, A. J. ^{[1
]}

Camargo, A. A. ^{[2
]}

Old, Lloyd J. ^{[1
]}

de Souza, S. J. ^{[2
]}

机构：

[1] Ludwig Inst Canc Res, New York Branch, New York, NY 10158 USA

[2] Ludwig Inst Canc Res, Sao Paulo Branch, BR-01323903 Sao Paulo, Brazil

[3] Univ Sao Paulo, Fac Med, BR-01246903 Sao Paulo, Brazil

[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14049900 Sao Paulo, Brazil

[5] Hosp Alemao Oswaldo Cruz, BR-01323903 Sao Paulo, Brazil

[6] Univ Sao Paulo, Inst Matemat & Estat, Dept Computacao, BR-05508090 Sao Paulo, Brazil

[7] Fluidigm Inc, San Francisco, CA 94080 USA

[8] Harvard Univ, Sch Med, Harvard Catalyst Lab Innovat Translat Technol, Boston, MA 02115 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 39期

基金：

美国国家卫生研究院;

关键词：

colorectal tumors; CT antigens; glioblastoma; transmembrane; tumor cell surface antigens; HUMAN BREAST; TUMOR; CANCER; EXPRESSION; RECEPTOR; IDENTIFICATION; PROLIFERATION; ENDOSIALIN; INHIBITION; TARGET;

D O I：

10.1073/pnas.0907939106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cell surface proteins are excellent targets for diagnostic and therapeutic interventions. By using bioinformatics tools, we generated a catalog of 3,702 transmembrane proteins located at the surface of human cells (human cell surfaceome). We explored the genetic diversity of the human cell surfaceome at different levels, including the distribution of polymorphisms, conservation among eukaryotic species, and patterns of gene expression. By integrating expression information from a variety of sources, we were able to identify surfaceome genes with a restricted expression in normal tissues and/or differential expression in tumors, important characteristics for putative tumor targets. A high-throughput and efficient quantitative real-time PCR approach was used to validate 593 surfaceome genes selected on the basis of their expression pattern in normal and tumor samples. A number of candidates were identified as potential diagnostic and therapeutic targets for colorectal tumors and glioblastoma. Several candidate genes were also identified as coding for cell surface cancer/testis antigens. The human cell surfaceome will serve as a reference for further studies aimed at characterizing tumor targets at the surface of human cells.

引用

页码：16752 / 16757

页数：6

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