Changing green leaf volatile biosynthesis in plants: An approach for improving plant resistance against both herbivores and pathogens

被引:216
作者
Shiojiri, Kaori
Kishimoto, Kyutaro
Ozawa, Rika
Kugimiya, Soichi
Urashimo, Soichi
Arimura, Genichiro
Horiuchi, Junichiro
Nishioka, Takaaki
Matsui, Kenji [1 ,2 ,3 ]
Takabayashi, Junji
机构
[1] Kyoto Univ, Ctr Ecol Res, Otsu, Shiga 5202113, Japan
[2] Japan Sci & Technol Corp, CREST, Kawaguchi, Saitama 3320012, Japan
[3] Yamaguchi Univ, Grad Sch Med, Dept Biol Chem, Fac Agr, Yamaguchi 7538518, Japan
关键词
Arabidopsis; hydroperoxicle lyase; tritrophic interactions; Cotesia glomerata; Botrytis cinerea;
D O I
10.1073/pnas.0607780103
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Green leaf volatiles (GLVs) are commonly emitted by green plants, and their production is drastically enhanced when they are under biotic stress. To clarify the ecological function of naturally emitted GLVs, we studied the response of Arabidopsis, whose GLV biosynthesis had been modified, when subjected to herbivory or a pathogenic infection. There was a significant increase in GLV production after herbivory by cabbage white butterfly larvae and pathogen (gray mold) infection in hydroperoxide lyase (HPL) sense Arabidopsis compared with WT controls. The HPL sense modification resulted in the plant being more attractive to the parasitic wasp Cotesia glomerata, leading to higher mortality of the herbivores. The HPL sense modification also resulted in greater inhibition of growth of the fungus. By contrast, HPL antisense Arabidopsis produced fewer GLVs, attracted fewer parasitoids, and was more susceptible to the pathogens than the WT control. These data show that (i) one of the ecological functions of GLV biosynthesis related to resistance against both herbivores and pathogens, and (ii) the genetic modification of GLV biosynthesis could be a unique approach for improving plant resistance against such biotic stresses.
引用
收藏
页码:16672 / 16676
页数:5
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