Controlled Co-delivery of Growth Factors through Layer-by-Layer Assembly of Core-Shell Nanofibers for Improving Bone Regeneration

被引：263

作者：

Cheng, Gu ^{[1
,2
]}

Yin, Chengcheng ^{[1
,2
]}

Tu, Hu ^{[3
]}

Jiang, Shan ^{[4
]}

Wang, Qun ^{[5
]}

Zhou, Xue ^{[6
]}

Xing, Xin ^{[1
,2
]}

Xie, Congyong ^{[1
,2
]}

Shi, Xiaowen ^{[3
]}

Du, Yuming ^{[3
]}

Deng, Hongbing ^{[3
]}

Li, Zubing ^{[1
,2
]}

机构：

[1] Wuhan Univ, Sch & Hosp Stomatol, State Key Lab Breeding Base Basic Sci Stomatol Hu, Wuhan 430079, Hubei, Peoples R China

[2] Wuhan Univ, Sch & Hosp Stomatol, Key Lab Oral Biomed, Minist Educ, Wuhan 430079, Hubei, Peoples R China

[3] Wuhan Univ, Hubei Key Lab Biomass Resource Chem & Environm Bi, Sch Resource & Environm Sci, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Hubei, Peoples R China

[4] Iowa State Univ, Dept Mat Sci & Engn, Ames, IA 50011 USA

[5] Iowa State Univ, Dept Chem & Biol Engn, Ames, IA 50011 USA

[6] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Publ Hlth, Wuhan 430030, Hubei, Peoples R China

来源：

ACS NANO | 2019年 / 13卷 / 06期

关键词：

growth factor; coaxial electrospinning; layer-by-layer; controlled release; bone defects; OSTEOGENIC DIFFERENTIATION; TISSUE-REPAIR; CELLS; DRUG; RELEASE; POLY(EPSILON-CAPROLACTONE); ANGIOGENESIS; PROMOTION; SYSTEM;

D O I：

10.1021/acsnano.8b06032

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

The regeneration of bone tissue is regulated by both osteogenic and angiogenic growth factors which are expressed in a coordinated cascade of events. The aim of this study was to create a dual growth factor-release system that allows for time-controlled release to facilitate bone regeneration. We fabricated core-shell SF/PCL/PVA nanofibrous mats using coaxial electrospinning and layer-by-layer (LBL) techniques, where bone morphogenetic protein 2 (BMP2) was incorporated into the core of the nanofibers and connective tissue growth factor (CTGF) was attached onto the surface. Our study confirmed the sustained release of BMP2 and a rapid release of CTGF. Both in vitro and in vivo experiments demonstrated improvements in bone tissue recovery with the dual-drug release system. In vivo studies showed improvement in bone regeneration by 43% compared with single BMP2 release systems. Time-controlled release enabled by the core-shell nanofiber assembly provides a promising strategy to facilitate bone healing.

引用

页码：6372 / 6382

页数：11

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