Alterations in the biosynthesis of lignin in transgenic plants with chimeric genes for 4-coumarate:coenzyme a ligase

被引:114
作者
Kajita, S [1 ]
Katayama, Y [1 ]
Omori, S [1 ]
机构
[1] TOKYO UNIV AGR & TECHNOL, GRAD SCH BIOAPPLICAT & SYST ENGN, FUCHU, TOKYO 183, JAPAN
关键词
4-coumarate:CoA ligase; genetic manipulation; lignin biosynthesis; transgenic tobacco;
D O I
10.1093/oxfordjournals.pcp.a029045
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The introduction of chimeric sense and antisense gene constructs for 4-coumarate:coenzyme A ligase into tobacco plants caused the reduction of the 4CL activity in the transgenic plants. In the transgenic plants, the cell walls of the xylem tissue in stems were brown and the molecular structure of lignin in the colored cell walls was dramatically different from that in the control plants. Analysis with different types of stain revealed that levels of cinnamyl aldehyde residues and syringyl units in lignin were depressed in the brownish cell walls. Furthermore, the lignin content in colored tissue was lower than that in the normal tissue. Our results indicate that 4CL has important roles in the determination of the composition and the amount of lignin in tobacco plants.
引用
收藏
页码:957 / 965
页数:9
相关论文
共 39 条
[1]   ALTERED LIGNIN COMPOSITION IN TRANSGENIC TOBACCO EXPRESSING O-METHYLTRANSFERASE SEQUENCES IN SENSE AND ANTISENSE ORIENTATION [J].
ATANASSOVA, R ;
FAVET, N ;
MARTZ, F ;
CHABBERT, B ;
TOLLIER, MT ;
MONTIES, B ;
FRITIG, B ;
LEGRAND, M .
PLANT JOURNAL, 1995, 8 (04) :465-477
[2]  
BECKERANDRE M, 1991, J BIOL CHEM, V266, P8551
[3]   BINARY AGROBACTERIUM VECTORS FOR PLANT TRANSFORMATION [J].
BEVAN, M .
NUCLEIC ACIDS RESEARCH, 1984, 12 (22) :8711-8721
[4]   THE FORMATION AND QUANTITY OF DIPHENYLMETHANE TYPE STRUCTURES IN RESIDUAL LIGNIN DURING KRAFT DELIGNIFICATION OF DOUGLAS-FIR [J].
CHIANG, VL ;
FUNAOKA, M .
HOLZFORSCHUNG, 1988, 42 (06) :385-391
[5]   4-COUMARATE, COENZYME-A LIGASE AND ISOPEROXIDASE EXPRESSION IN ZINNIA MESOPHYLL-CELLS INDUCED TO DIFFERENTIATE INTO TRACHEARY ELEMENTS [J].
CHURCH, DL ;
GALSTON, AW .
PLANT PHYSIOLOGY, 1988, 88 (03) :679-684
[6]   STRUCTURE AND ELICITOR OR UV-LIGHT-STIMULATED EXPRESSION OF 2 4-COUMARATE-COA LIGASE GENES IN PARSLEY [J].
DOUGLAS, C ;
HOFFMANN, H ;
SCHULZ, W ;
HAHLBROCK, K .
EMBO JOURNAL, 1987, 6 (05) :1189-1195
[7]   EXONIC SEQUENCES ARE REQUIRED FOR ELICITOR AND LIGHT ACTIVATION OF A PLANT DEFENSE GENE, BUT PROMOTER SEQUENCES ARE SUFFICIENT FOR TISSUE SPECIFIC EXPRESSION [J].
DOUGLAS, CJ ;
HAUFFE, KD ;
ITESMORALES, ME ;
ELLARD, M ;
PASZKOWSKI, U ;
HAHLBROCK, K ;
DANGL, JL .
EMBO JOURNAL, 1991, 10 (07) :1767-1775
[8]   MODIFICATION OF LIGNIN BIOSYNTHESIS IN TRANSGENIC NICOTIANA THROUGH EXPRESSION OF AN ANTISENSE O-METHYLTRANSFERASE GENE FROM POPULUS [J].
DWIVEDI, UN ;
CAMPBELL, WH ;
YU, J ;
DATLA, RSS ;
BUGOS, RC ;
CHIANG, VL ;
PODILA, GK .
PLANT MOLECULAR BIOLOGY, 1994, 26 (01) :61-71
[9]  
FUKUSHIMA K, 1994, MOKUZAI GAKKAISHI, V40, P958
[10]   ISOENZYMES OF HYDROXYCINNAMATE - COA LIGASE FROM POPLAR STEMS PROPERTIES AND TISSUE DISTRIBUTION [J].
GRAND, C ;
BOUDET, A ;
BOUDET, AM .
PLANTA, 1983, 158 (03) :225-229