Separation and determination of homovanillic acid and vanillylmandelic acid by capillary electrophoresis with electrochemical detection

被引：36

作者：

Li, XJ ^{[1
]}

Jin, WR ^{[1
]}

Weng, QF ^{[1
]}

机构：

[1] Shandong Univ, Sch Chem & Chem Engn, State Key Lab Microbial Technol, Jinan 250100, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2002年 / 461卷 / 01期

基金：

中国国家自然科学基金;

关键词：

capillary electrophoresis; electrochemical detection; homovanillic acid; vanillylmandelic acid; urine;

D O I：

10.1016/S0003-2670(02)00241-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method of separation and determination of homovanillic acid (HVA) and vanillylmandelic acid (VMA) was developed based on capillary zone electrophoresis/amperometric detection with high sensitivity, good resolution and selectivity. In order to achieve complete separation and good response, several factors including pH, buffer concentration, separation voltage, detection potential and the length of separation capillary, were studied in detail. The method has been used to determine both HVA and VMA in human urine. Uric acid (UA) in human urine did not interference with their determination. The limit of detection of the method was 1.3 x 10(-6) mol/l (1.4 fmol) for HVA and 7.9 x 10(-7) mol/l (0.87 fmol) for VMA at a signal-to-noise ratio of 3. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：123 / 130

页数：8

共 19 条

[1] MODIFICATION OF A TUNABLE UV-VISIBLE CAPILLARY ELECTROPHORESIS DETECTOR FOR SIMULTANEOUS ABSORBENCY AND FLUORESCENCE DETECTION - PROFILING OF BODY-FLUIDS FOR DRUGS AND ENDOGENOUS COMPOUNDS [J].