Brachyury is a target gene of the Wnt/β-catenin signaling pathway

被引：232

作者：

Arnold, SJ ^{[1
]}

Stappert, J ^{[1
]}

Bauer, A ^{[1
]}

Kispert, A ^{[1
]}

Herrmann, BG ^{[1
]}

Kemler, R ^{[1
]}

机构：

[1] Max Planck Inst Immunbiol, D-79108 Freiburg, Germany

来源：

MECHANISMS OF DEVELOPMENT | 2000年 / 91卷 / 1-2期

关键词：

mouse embryonic development; mesoderm formation; embryonic stem cells; Wnt/beta-catenin signaling pathway; Brachyury;

D O I：

10.1016/S0925-4773(99)00309-3

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To identify target genes of the Wnt/beta-catenin signaling pathway in early mouse embryonic development we have established a co-culture system consisting of NIH3T3 fibroblasts expressing different Wnts as feeder layer cells and embryonic stem (ES) cells expressing a green fluorescent protein (GFP) reporter gene transcriptionally regulated by the TCF/beta-catenin complex. ES cells specifically respond to Wnt signal as monitored by GFP expression. In GFP-positive ES cells we observe expression of Brachyury. Two TCF binding sites located in a 500 bp Brachyury promoter fragment bind the LEF-1/beta-catenin complex and respond specifically to beta-catenin-dependent transactivation. From these results we conclude that Brachyury is a target gene for Wnt/beta-catenin signaling. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：249 / 258

页数：10

共 53 条

[1] beta-catenin is a target for the ubiquitin-proteasome pathway [J].