Ultrasensitive, direct detection of a specific DNA sequence of Bacillus anthracis in solution

被引:30
作者
Castro, A [1 ]
Okinaka, RT [1 ]
机构
[1] Univ Calif Los Alamos Natl Lab, Los Alamos, NM 87545 USA
关键词
D O I
10.1039/a908067h
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A very fast and ultrasensitive method has been developed for the detection and quantitation of specific nucleic and sequences of bacterial origin in solution. The method is based on a two-color, single fluorescent molecule detection technique developed in our laboratory. The technique was applied to the detection of Bacillus anthracis DNA in solution.
引用
收藏
页码:9 / 11
页数:3
相关论文
共 16 条
[1]   AMPLIFICATION OF NUCLEIC-ACIDS BY POLYMERASE CHAIN-REACTION (PCR) AND OTHER METHODS AND THEIR APPLICATIONS [J].
BEJ, AK ;
MAHBUBANI, MH ;
ATLAS, RM .
CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1991, 26 (3-4) :301-334
[2]   Single-molecule detection of specific nucleic acid sequences in unamplified genomic DNA [J].
Castro, A ;
Williams, JGK .
ANALYTICAL CHEMISTRY, 1997, 69 (19) :3915-3920
[3]   FLUORESCENCE DETECTION AND SIZE MEASUREMENT OF SINGLE DNA-MOLECULES [J].
CASTRO, A ;
FAIRFIELD, FR ;
SHERA, EB .
ANALYTICAL CHEMISTRY, 1993, 65 (07) :849-852
[4]   SINGLE-MOLECULE ELECTROPHORESIS [J].
CASTRO, A ;
SHERA, EB .
ANALYTICAL CHEMISTRY, 1995, 67 (18) :3181-3186
[5]   ERRORS IN THE POLYMERASE CHAIN-REACTION [J].
DUNNING, AM ;
TALMUD, P ;
HUMPHRIES, SE .
NUCLEIC ACIDS RESEARCH, 1988, 16 (21) :10393-10393
[6]  
Greenberg H., 1985, STANDARD METHODS EXA
[7]  
KWOH S, 1989, NATURE, V339, P237
[8]   ANTHRAX TOXIN EDEMA FACTOR - A BACTERIAL ADENYLATE-CYCLASE THAT INCREASES CYCLIC-AMP CONCENTRATIONS IN EUKARYOTIC CELLS [J].
LEPPLA, SH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (10) :3162-3166
[9]  
LEW D, 1995, PRINCIPLES PRACTICE, P1885
[10]   Utility of 16S-23S rRNA spacer region methodology: how similar are interspace regions within a genome and between strains for closely related organisms? [J].
Nagpal, ML ;
Fox, KF ;
Fox, A .
JOURNAL OF MICROBIOLOGICAL METHODS, 1998, 33 (03) :211-219