Isopentenyl diphosphate isomerase and prenyl transferase activities in bottom fraction and C-serum from Hevea latex

被引:27
作者
Tangpakdee, J
Tanaka, Y
Ogura, K
Koyama, T
Wititsuwannakul, R
Wititsuwannakul, D
Asawatreratanakul, K
机构
[1] TOKYO UNIV AGR & TECHNOL, FAC TECHNOL, DEPT APPL CHEM, KOGANEI, TOKYO 184, JAPAN
[2] TOHOKU UNIV, INST CHEM REACT SCI, SENDAI, MIYAGI 980, JAPAN
[3] PRINCE SONGKLA UNIV, FAC SCI, DEPT BIOCHEM, SONGKHLA 90112, THAILAND
[4] MAHIDOL UNIV, FAC SCI, DEPT BIOCHEM, BANGKOK 10400, THAILAND
关键词
Hevea brasiliensis; latex; fresh bottom fraction; freeze-dried bottom fraction; C-serum; polyprenyl diphosphate; isopentenyl diphosphate isomerase; polyprenyltransferases; RUBBER ELONGATION-FACTOR; PARTHENIUM-ARGENTATUM; CIS-POLYISOPRENE; ISOPRENE UNITS; BIOSYNTHESIS; BRASILIENSIS; PARTICLES; GUAYULE; PROTEIN;
D O I
10.1016/S0031-9422(96)00837-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The isomerization of isopentenyl diphosphate (IDP) to dimethylallyl diphosphate (DMADP), and the formation of oligoprenyl diphosphates as well as polyprenyl diphosphates, catalyzed by IDP-isomerase and polyprenyl transferases, respectively, were observed on incubation of a mixture of C-14-IDP and fresh bottom fraction (BF) (separated from centrifuged fresh Hevea latex) which had been preincubated at 4 degrees overnight. The incubation of a freeze-dried BF with C-14 IDP gave DMADP, farnesyl diphosphate (FDP) and polyprenyl diphosphate as predominant products, while that of fresh BE gave C-20- and C-40-diphosphates, as well as rubber as main products, FDP was not detected in the incubation of the fresh BF, suggesting that it was consumed as a primer for the formation of rubber and polyprenols. Direct evidence was obtained to show the presence of IDP-isomerase and polyprenyl transferase activities in the BF of fresh latex. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码:261 / 267
页数:7
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