Contraceptive effect of sperm-agglutinating monoclonal antibodies in rabbits

被引：34

作者：

Castle, PE ^{[1
]}

Whaley, KJ ^{[1
]}

Hoen, TE ^{[1
]}

Moench, TR ^{[1
]}

Cone, RA ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV, THOMAS C JENKINS DEPT BIOPHYS, BALTIMORE, MD 21218 USA

来源：

BIOLOGY OF REPRODUCTION | 1997年 / 56卷 / 01期

关键词：

D O I：

10.1095/biolreprod56.1.153

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Immune infertility in humans correlates clinically with the presence of anti-sperm antibodies that trap (agglutinate) sperm tn semen and cervical mucus. To test whether sperm-agglutinating antibodies can be effective contraceptive agents, several mouse anti-rabbit sperm (MARS) sperm-agglutinating monoclonal antibodies (mAbs) were developed that rapidly and completely agglutinate sperm: MARS-M3 (IgM), MARS-G16 (IgG(3)), and MARS-G17 (IgG(3)). Contraceptive efficacy of these mAbs was tested by mixing the mAb with 0.1 ml semen (similar to 1/5 of a whole ejaculate) immediately before artificially inseminating rabbits paracervically. This paracervical dose of semen provided a rigorous test since it delivered several thousand times more fertilizing doses than does a human ejaculate. All of the mAbs were contraceptively effective, and MARS-G16 reduced the number of fetuses per animal by 88% and 95% with doses of 150 mu g and 2 mg, respectively. The contraceptive efficacy of the MARS mAbs in the rabbit suggests that human sperm-agglutinating mAbs may be effective agents for vaginal contraception in humans.

引用

页码：153 / 159

页数：7

共 44 条

[1] ALEXANDER NJ, 1984, FERTIL STERIL, V41, P433
[2] MONOCLONAL-ANTIBODIES TO HUMAN TROPHOBLAST AND SPERM ANTIGENS - REPORT OF 2 WHO-SPONSORED WORKSHOPS, JUNE 30, 1986 - TORONTO, CANADA
ANDERSON, DJ
JOHNSON, PM
ALEXANDER, NJ
JONES, WR
GRIFFIN, PD
[J]. JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 1987, 10 (03) : 231 - 257
[3] BERZOFSKY JA, 1989, FUNDAMENTAL IMMUNOLO, P337
[4] BRONSON R, 1984, FERTIL STERIL, V42, P171
[5] BRONSON RA, 1984, FERTIL STERIL, V41, P609
[6] Bronson RA, 1990, INFERTILITY COMPREHE, P217
[7] CASTLE P, 1993, BIOL REPROD, V48, pS181
[8] CASTLE PE, 1991, J ANDROL, V12, pS27
[9] CLARKE GN, 1988, AM J REPROD IMMUNOL, V17, P65
[10] MONOCLONAL-ANTIBODIES FOR REPRODUCTIVE HEALTH .1. PREVENTING SEXUAL TRANSMISSION OF DISEASE AND PREGNANCY WITH TOPICALLY APPLIED ANTIBODIES
CONE, RA
WHALEY, KJ
[J]. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 1994, 32 (02): : 114 - 131

← 1 2 3 4 5 →