Measurement of genome wide DNA methylation by reversed-phase high-performance liquid chromatography

被引：89

作者：

Ramsahoye, BH ^{[1
]}

机构：

[1] Western Gen Hosp, Dept Haematol, John Hughes Bennet Lab, Edinburgh EH4 2XU, Midlothian, Scotland

来源：

METHODS | 2002年 / 27卷 / 02期

关键词：

D O I：

10.1016/S1046-2023(02)00069-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method to determine the extent of cytosine methylation in DNA is described. The technique involves the enzymatic hydrolysis of DNA to its deoxyribonucleotide components and subsequent separation and quantification of the nucleotides by isocratic reversed-phase high-performance liquid chromatography (HPLC), The system gives highly reproducible results and, under suitable conditions, is capable of measuring 5-methylcytosine levels in as little as 1 mug of DNA. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：156 / 161

页数：6

共 5 条

[1] QUANTITATIVE-DETERMINATION OF 5-METHYLCYTOSINE IN DNA BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].