Changes in mRNA levels of fibrinogen subunit polypeptides in rats defibrinogenated with Batroxobin

被引：9

作者：

Iwai, S ^{[1
]}

Okazaki, M ^{[1
]}

Kiuchi, Y ^{[1
]}

Oguchi, K ^{[1
]}

机构：

[1] Showa Univ, Sch Med, Dept Pharmacol, Shinagawa Ku, Tokyo 1428555, Japan

来源：

THROMBOSIS RESEARCH | 1999年 / 96卷 / 06期

关键词：

Batroxobin; fibrinogen; mRNA; reverse transcription-polymerase chain reaction; coagulation factor; rats;

D O I：

10.1016/S0049-3848(99)00135-8

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Batroxobin is a snake venom that is a thrombin-like enzyme used for clinical treatment. We analyzed hepatic mRNA levels for fibrinogen subunit polypeptides and prothrombin by reverse transcription-polymerase chain reaction as well as coagulation and fibrinolysis factors in plasma 1, 3, 5 and 24 hours after Batroxobin treatment (3 BU/100 g) in rats. The mRNA levels of alpha- and beta-chains of fibrinogen were significantly increased with decreases in plasma fibrinogen, alpha(2)-plasmin inhibitor, and plasminogen levels, while the mRNA levels for prothrombin remained unchanged. These results suggest that fibrinogen mRNA synthesis is regulated by plasma fibrinogen levels in Batroxobin-induced defibrinogenated rats. (C) 1999 Elsevier Science Ltd, All rights reserved.

引用

页码：421 / 426

页数：6

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