Preparative separation of saponins from the Luffa cylindeica (L.) Roem. by slow rotary countercurrent chromatography

被引：15

作者：

Du, Qizhen ^{[1
]}

Gao, Shijun ^{[1
]}

机构：

[1] Zhejiang Gongshang Univ, Inst Food & Biol Engn, Hangzhou 310035, Peoples R China

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2006年 / 29卷 / 16期

关键词：

Luffa cyclindrica; saponins; slow rotary countercurrent chromatography; preparative isolation; Lucyoside Q; Lucyoside H;

D O I：

10.1080/10826070600864924

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Slow rotary countercurrent chromatography (SRCCC) was used for the separation of saponins from towel gourd crude extract (Luffa cyclindria Roem.) with a biphasic solvent system composed of chloroform-methanol-water (13:7:8, v/v). In each separation of SRCCC, 3 g crude saponins sample yielded 357 mg Lucyoside Q (1) and 213 mg Lucyoside H (2). The structures of the two compounds were confirmed by means of ESI-MS, H-1- and C-13-NMR.

引用

页码：2451 / 2456

页数：6

共 11 条

[1]

Low speed rotary countercurrent chromatography using a convoluted multilayer helical tube for industrial separation [J].