An SECM detection scheme with improved sensitivity and lateral resolution: Detection of galactosidase activity with signal amplification by glucose dehydrogenase

被引：28

作者：

Zhao, C ^{[1
]}

Wittstock, G ^{[1
]}

机构：

[1] Carl Von Ossietzky Univ Oldenburg, Fac Math & Nat Sci, D-26111 Oldenburg, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 32期

关键词：

electrochemistry; enzyme catalysis; interfaces; scanning probe microscopy; SECM;

D O I：

10.1002/anie.200454261

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An enzymatic amplification cycle is used in a novel detection scheme for scanning electrochemical microscopy (SECM). An immobilized enzyme system comprising galactosidase and PQQ-dependent glucose dehydrogenase was imaged by SECM with high sensitivity and high lateral resolution. pQQ = pyrroloquinolinequinone.

引用

页码：4170 / 4172

页数：3

共 20 条

[1] Quinoprotein-catalysed reactions [J].

Anthony, C .

BIOCHEMICAL JOURNAL, 1996, 320 :697-711

[2]

Bard A.J., 2001, Scanning Electrochemical Microscopy

[3] CHEMICAL IMAGING OF SURFACES WITH THE SCANNING ELECTROCHEMICAL MICROSCOPE [J].

BARD, AJ ;

FAN, FRF ;

PIERCE, DT ;

UNWIN, PR ;

WIPF, DO ;

ZHOU, FM .

SCIENCE, 1991, 254 (5028) :68-74

[4] Scanning electrochemical microscopy: beyond the solid/liquid interface [J].

Barker, AL ;

Gonsalves, M ;

Macpherson, JV ;

Slevin, CJ ;

Unwin, PR .

ANALYTICA CHIMICA ACTA, 1999, 385 (1-3) :223-240

[5] An enzyme flow immunoassay that uses β-galactosidase as the label and a cellobiose dehydrogenase biosensor as the label detector [J].

Burestedt, E ;

Nistor, C ;

Schagerlöf, U ;

Emnéus, J .

ANALYTICAL CHEMISTRY, 2000, 72 (17) :4171-4177

[6] The PQQ story [J].

Duine, JA .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 88 (03) :231-236

[7] A method for the design and study of enzyme microstructures formed by means of a flow-through microdispenser [J].

Gáspár, S ;

Mosbach, M ;

Wallman, L ;

Laurell, T ;

Csoregi, E ;

Schuhmann, W .

ANALYTICAL CHEMISTRY, 2001, 73 (17) :4254-4261

[8]

HNATOWICH DJ, 1987, J NUCL MED, V28, P1294

[9] LATERAL DEPOSITION OF POLYPYRROLE LINES BY MEANS OF THE SCANNING ELECTROCHEMICAL MICROSCOPE [J].

KRANZ, C ;

LUDWIG, M ;

GAUB, HE ;

SCHUHMANN, W .

ADVANCED MATERIALS, 1995, 7 (01) :38-40

[10] HIGH-CURRENT DENSITY WIRED QUINOPROTEIN GLUCOSE-DEHYDROGENASE ELECTRODE [J].

LING, Y ;

HAMMERLE, M ;

OLSTHOORN, AJJ ;

SCHUHMANN, W ;

SCHMIDT, HL ;

DUINE, JA ;

HELLER, A .

ANALYTICAL CHEMISTRY, 1993, 65 (03) :238-241

← 1 2 →