Effects of Resveratrol on H2O2-Induced Apoptosis and Expression of SIRTs in H9c2 Cells

被引：67

作者：

Yu, Wei ^{[1
]}

Fu, Yu-Cai ^{[2
]}

Zhou, Xiao-Hui ^{[2
]}

Chen, Chun-Juan ^{[1
]}

Wang, Xin ^{[1
]}

Lin, Rui-Bo ^{[1
]}

Wang, Wei ^{[1
]}

机构：

[1] Shantou Univ, Coll Med, Affiliated Hosp 1, Dept Cardiol, Shantou 515041, Peoples R China

[2] Shantou Univ, Coll Med, Lab Cell Senescence, Shantou 515041, Peoples R China

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2009年 / 107卷 / 04期

关键词：

RESVERATROL; SIRTs; OXIDATIVE STRESS; CARDIOMYOCYTE APOPTOSIS; CASPASE-3; TRANSCRIPTION FACTORS; HISTONE DEACETYLASE; SIR2-LIKE PROTEINS; OXIDATIVE STRESS; CARDIOMYOCYTES; SIRTUINS; PATHWAY; RESTRICTION; ACTIVATION; RESISTANCE;

D O I：

10.1002/jcb.22169

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Resveratrol, a polyphenol found in fruits, has been demonstrated to activate Sir2. Though many studies have demonstrated that resveratrol can activate SIRT1, whether it has effect on other sirtuins (SIRT2-7) are unknown. The present study shows that exposure of H9c2 cells to 50 mu M H2O2 for 6 h caused a significant increase in apoptosis, as evaluated by TUNEL and flow cytometry (FCM), but pretreatment of resveratrol (20 mu M) eliminated H2O2-induced apoptosis. Resveratrol also prevented H2O2-induced caspase-3 activation. Exposure of cells to resveratrol caused rapid activation of SIRT1,3,4,7. Sirtuin inhibitor, nicotinamide (20 mM) attenuated resveratrol's inhibitory effect on cell apoptosis and caspase-3 activity. These results suggest that resveratrol protects cardiomyocytes from H2O2-induced apoptosis by activating SIRT1,3,4,7. J. Cell. Biochem. 107: 741-747, 2009. (C) 2009 Wiley-Liss, Inc.

引用

页码：741 / 747

页数：7

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