Protective effects of proline against cadmium toxicity in micropropagated hyperaccumulator, Solanum nigrum L.

被引：148

作者：

Xu, Jin ^{[1
]}

Yin, HengXia ^{[1
]}

Li, Xia ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Agr Resources Res, Shijiazhuang 050021, Peoples R China

来源：

PLANT CELL REPORTS | 2009年 / 28卷 / 02期

关键词：

In vitro culture; Growth regulator; Heavy metal tolerance; Antioxidant systems; SUPEROXIDE-DISMUTASE; MINERAL-NUTRITION; ACCUMULATION; GROWTH; SEEDLINGS; STRESS; PHYTOEXTRACTION; GLUTATHIONE; PEROXIDASE; CATALASE;

D O I：

10.1007/s00299-008-0643-5

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Solanum nigrum is a newly discovered Cd-hyperaccumulator. In the present study, the protective effects of proline against cadmium toxicity of callus and regenerated shoots of S. nigrum are investigated based on a high frequency in vitro shoot regeneration system. Proline pretreatment reduces the reactive oxygen species levels and protects the plasma membrane integrity of callus under cadmium stress, and therefore improves the cadmium tolerance in S. nigrum. Inductively coupled plasma mass spectroscopy analysis shows that exogenous proline increases the cadmium accumulation in callus and regenerated shoots of S. nigrum. Further analysis indicates that the improvement of cadmium tolerance caused by proline pretreatment is correlated with an increase of superoxide dismutase and catalase activity and intracellular total glutathione content. The interaction between proline and enzymic or non-enzymic antioxidants is discussed.

引用

页码：325 / 333

页数：9

共 40 条

[1] CHANGES IN ISOZYME PROFILES OF CATALASE, PEROXIDASE, AND GLUTATHIONE-REDUCTASE DURING ACCLIMATION TO CHILLING IN MESOCOTYLS OF MAIZE SEEDLINGS [J].