Expression of the fibroblast growth factor-2 isoforms and the FGF receptor 1-4 transcripts in the rat model system of Parkinson's disease

被引：28

作者：

Claus, P ^{[1
]}

Werner, S ^{[1
]}

Timmer, M ^{[1
]}

Grothe, C ^{[1
]}

机构：

[1] Hannover Med Sch, Dept Neuroanat, D-30625 Hannover, Germany

来源：

NEUROSCIENCE LETTERS | 2004年 / 360卷 / 03期

关键词：

fibroblast growth factor; 6-hydroxydopamine; lesion; substantia nigra; striatum;

D O I：

10.1016/j.neulet.2004.01.046

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Basic fibroblast growth factor (FGF)-2 occurs in different isoforms representing different translation products of a single mRNA. We have previously shown that the high molecular weight FGF-2 isoforms (21, 23 kD) stimulated survival- and neurite-promoting activities and protective effects on Cultured embryonic dopaminergic (DA) neurons of the substantia nigra (Neuroscience 100 (2000) 73). In this study the expression of FGF-2 isoforms in the striatum and substantia nigra was analyzed by Western blot in adult intact rats and following complete unilateral 6-hydroxydopamine (6-OHDA) lesion. In intact rats, all three FGF-2 isoforms (18, 21, 23 kD) are expressed. Neurotoxin-mediated lesion of nigral DA neurons revealed no change of the FGF-2 isoform expression pattern in the nigrostriatal system. Additionally, the FGF receptors 1, 2 and 3 are expressed in these tissues and displayed no alterations after 6-OHDA injection as demonstrated by RT-PCR. The presence of all three FGF-2 isoforms and the FGFR 1-3, together with the previous demonstrated neurotrophic effects of FGF-2 on dopaminergic neurons, suggest a physiological function of the FGF-2 isoforms in the nigrostriatal system. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

引用

页码：117 / 120

页数：4

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