Characterization of Human Skeletal Muscle Biopsy Samples Using Shotgun Proteomics

被引：60

作者：

Parker, Kenneth C. ^{[1
,2
,3
]}

Walsh, Ronan J. ^{[2
,3
]}

Salajegheh, Mohammad ^{[2
,3
]}

Amato, Anthony A. ^{[3
]}

Krastins, Bryan ^{[1
]}

Sarracino, David A. ^{[1
]}

Greenberg, Steven A. ^{[2
,3
]}

机构：

[1] Harvard Partners Ctr Genet & Genom, Cambridge, MA USA

[2] Harvard Univ, Brigham & Womens Hosp, Dept Neurol, Sch Med, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Childrens Hosp Boston, Informat Program, Boston, MA USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 07期

关键词：

muscle; biopsy; shotgun; abundance; protein correlation profiling; sarcomere; workflow comparison; COMPLEX; PROTEINS; TANDEM; DEPTH; FORM;

D O I：

10.1021/pr800873q

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

We characterized the human muscle proteome by studying muscle biopsy specimens through four different workflows, using 1 or 2D peptide separation, SDS gels, or differential solubilization. By performing MS/MS analyses of 178 4-h LC separations derived from 31 patients, we identified more than 2000 proteins, and determined how 370 very abundant proteins behave upon differential solubilization. The resulting semiquantitative database should serve as a resource for muscle biochemistry.

引用

页码：3265 / 3277

页数：13

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