Biosynthesis of polysaccharide alginate by Azotobacter vinelandii in a bubble column

被引：5

作者：

Asami, K ^{[1
]}

Aritomi, T ^{[1
]}

Tan, YS ^{[1
]}

Ohtaguchi, K ^{[1
]}

机构：

[1] Tokyo Inst Technol, Dept Chem Engn, Meguro Ku, Tokyo 1528552, Japan

来源：

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN | 2004年 / 37卷 / 08期

关键词：

Azotobacter vinelandii; bubble column bioreactor; microbial alginate;

D O I：

10.1252/jcej.37.1050

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Polysaccharide alginate, which is composed of mannuronic acid (M) and guluronic acid (G), is used as a gel-former and viscosifier in a wide range of applications. The properties of gel-forming, water-binding, and immunogenicity are influenced by the fraction of M- and G-monomers in the alginate. In this study, we engineered an alginate synthesis using culture of Azotobacter vinelandii IAM 9046. The effect of growth conditions on the cell concentration, alginate synthesis, fraction of monomers, etc., were investigated. As a result, alginate with a high content of serial G-monomers was produced in a bubble column reactor. It is our goal to control the monomer fraction of alginate that is required for medical and industrial applications.

引用

页码：1050 / 1055

页数：6

共 22 条

[1]

ANNISON G, 1984, APPL MICROBIOL BIOT, V19, P321

[2]

BRIVONESE AC, 1989, APPL MICROBIOL BIOT, V30, P97

[3]

Calafiore R, 1988, Minerva Endocrinol, V13, P287

[4] BACTERIAL ALGINATE PRODUCED BY A MUTANT OF AZOTOBACTER-VINELANDII [J].

CHEN, WP ;

CHEN, JY ;

CHANG, SC ;

SU, CL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1985, 49 (03) :543-546

[5] OPTIMAL CONDITIONS FOR ALGINATE PRODUCTION BY AZOTOBACTER-VINELANDII [J].

CLEMENTI, F ;

FANTOZZI, P ;

MANCINI, F ;

MORESI, M .

ENZYME AND MICROBIAL TECHNOLOGY, 1995, 17 (11) :983-988

[6] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].

DUBOIS, M ;

GILLES, KA ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356

[7] THE INVOLVEMENT OF CD14 IN STIMULATION OF CYTOKINE PRODUCTION BY URONIC-ACID POLYMERS [J].

ESPEVIK, T ;

OTTERLEI, M ;

SKJAKBRAEK, G ;

RYAN, L ;

WRIGHT, SD ;

SUNDAN, A .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1993, 23 (01) :255-261

[8] PRODUCTION AND CHARACTERIZATION OF SLIME POLYSACCHARIDE OF PSEUDOMONAS-AERUGINOSA [J].

EVANS, LR ;

LINKER, A .

JOURNAL OF BACTERIOLOGY, 1973, 116 (02) :915-924

[9] THE EFFECT OF THE CONTENT OF D-MANNURONIC ACID AND L-GULURONIC ACID BLOCKS IN ALGINATES ON ANTITUMOR-ACTIVITY [J].

FUJIHARA, M ;

NAGUMO, T .

CARBOHYDRATE RESEARCH, 1992, 224 :343-347

[10] URONIC ACID SEQUENCE IN ALGINATE FROM DIFFERENT SOURCES [J].

HAUG, A ;

LARSEN, B ;

SMIDSROD, O .

CARBOHYDRATE RESEARCH, 1974, 32 (02) :217-225

← 1 2 3 →