IgE Receptor-Mediated Mast-Cell Renin Release

Renin is a newly discovered constituent of mast cells. Given that mast cells play a major role in IgE-mediated allergic hypersensitivity, we investigated whether activation of the high-affinity IgE receptor Fc epsilon RI elicits release of mast-cell renin. Cross-linking of Fc epsilon RI on the surface of mature bone marrow derived mast cells elicited release of enzymatically active renin protein. The angiotensin I forming activity of the renin protein was completely blocked by the selective renin inhibitor BILA 2157, which excludes formation of angiotensin I by proteases other than renin. Fc epsilon RI-mediated mast-cell renin release was inhibited by dexamethasone and potentiated by the proinflammatory mediator PGE(2). Furthermore, cross-linking of mast-cell FceRI in ex vivo murine hearts passively sensitized with monoclonal anti-DNP IgE also resulted in mast-cell degranulation and overflow of renin. Our findings indicate that IgE-mediated allergic hypersensitivity provokes release of renin from both cultured and resident cardiac mast cells, a process likely to be exacerbated in a chronic inflammatory background. Given the widespread distribution of mast cells, and the presence of angiotensinogen and angiotensin-converting enzyme in many tissues, renin release in immediate hypersensitivity reactions could result in local angiotensin II generation and multiorgan dysfunctions.