Highly sensitive high-performance liquid chromatographic tandem mass spectrometric method for the analysis of dextromethorphan in human plasma

被引：21

作者：

Eichhold, TH ^{[1
]}

Quijano, M ^{[1
]}

Seibel, WL ^{[1
]}

Cruze, CA ^{[1
]}

Dobson, RLM ^{[1
]}

Wehmeyer, KR ^{[1
]}

机构：

[1] PROCTER & GAMBLE PHARMACEUT,MASON,OH 45040

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 698卷 / 1-2期

关键词：

dextromethorphan;

D O I：

10.1016/S0378-4347(97)00308-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A stable-isotope-dilution HPLC-tandem mass spectrometry-based method was developed for the determination of dextromethorphan in human plasma. Plasma samples were prepared for analysis by solid-phase extraction on octadecylsilane extraction cartridges. Dextromethorphan and the deuterium-labeled dextromethorphan internal standard were chromatographed on a short reversed-phase column and detected by a selected-reaction-monitoring scheme. Linear standard curves were obtained over three orders of magnitude and the limit of quantitation for dextromethorphan was 50 pg/ml, using a 1-ml plasma sample. The combination of HPLC and electrospray tandem mass spectrometry resulted in a rapid, selective and sensitive method for the analysis of dextromethorphan in plasma. The method was applied for the evaluation of the pharmacokinetic profile of dextromethorphan in human volunteers following peroral administration. (C) 1997 Elsevier Science B.V.

引用

页码：147 / 154

页数：8

共 17 条

[1] DETERMINATION OF DEXTROMETHORPHAN IN BIOLOGICAL-FLUIDS BY LIQUID-CHROMATOGRAPHY BY USING SEMI-MICROBORE COLUMNS [J].