Controlled, scalable embryonic stem cell differentiation culture

被引:212
作者
Dang, SM
Gerecht-Nir, S
Chen, J
Itskovitz-Eldor, J
Zandstra, PW
机构
[1] Univ Toronto, Dept Chem Engn & Appl Chem, Inst Biomat & Biomed Engn, Toronto, ON M5S 3G9, Canada
[2] Technion Israel Inst Technol, Fac Med, Haifa, Israel
[3] Rambam Med Ctr, Dept Obstet & Gynecol, IL-31096 Haifa, Israel
[4] Technion Israel Inst Technol, Biotechnol Interdisciplinary Unit, Haifa, Israel
关键词
stem cell culture; encapsulation; embryonic stem cells; embryoid body; scalable;
D O I
10.1634/stemcells.22-3-275
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Embryonic stem (ES) cells are of significant interest as a renewable source of therapeutically useful cells. ES cell aggregation is important for both human and mouse embryoid body (EB) formation and the subsequent generation of ES cell derivatives. Aggregation between EBs (agglomeration), however, inhibits cell growth and differentiation in stirred or high-cell-density static cultures. We demonstrate that the agglomeration of two EBs is initiated by E-cadherin-mediated cell attachment and followed by active cell migration. We report the development of a technology capable of controlling cell-cell interactions in scalable culture by the mass encapsulation of ES cells in size-specified agarose capsules. When placed in stirred-suspension bioreactors, encapsulated ES cells can be used to produce scalable quantities of hematopoietic progenitor cells in a controlled environment.
引用
收藏
页码:275 / 282
页数:8
相关论文
共 29 条
[1]  
Adelman DM, 2000, ADV EXP MED BIOL, V475, P275
[2]   Expression of cell adhesion molecules during human preimplantation embryo development [J].
Bloor, DJ ;
Metcalfe, AD ;
Rutherford, A ;
Brison, DR ;
Kimber, SJ .
MOLECULAR HUMAN REPRODUCTION, 2002, 8 (03) :237-245
[3]   Differentiation of pluripotent embryonic stem cells into cardiomyocytes [J].
Boheler, KR ;
Czyz, J ;
Tweedie, D ;
Yang, HT ;
Anisimov, SV ;
Wobus, AM .
CIRCULATION RESEARCH, 2002, 91 (03) :189-201
[4]   Efficiency of embryoid body formation and hematopoietic development from embryonic stem cells in different culture systems [J].
Dang, SM ;
Kyba, M ;
Perlingeiro, R ;
Daley, GQ ;
Zandstra, PW .
BIOTECHNOLOGY AND BIOENGINEERING, 2002, 78 (04) :442-453
[5]  
Dee KU, 1997, BIOTECHNOL BIOENG, V54, P191, DOI 10.1002/(SICI)1097-0290(19970505)54:3&lt
[6]  
191::AID-BIT1&gt
[7]  
3.0.CO
[8]  
2-A
[9]   ESTABLISHMENT IN CULTURE OF PLURIPOTENTIAL CELLS FROM MOUSE EMBRYOS [J].
EVANS, MJ ;
KAUFMAN, MH .
NATURE, 1981, 292 (5819) :154-156
[10]   Oxygen supply and oxygen-dependent gene expression in differentiating embryonic stem cells [J].
Gassmann, M ;
Fandrey, J ;
Bichet, S ;
Wartenberg, M ;
Marti, HH ;
Bauer, C ;
Wenger, RH ;
Acker, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (07) :2867-2872