Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation

被引:255
作者
Kurasawa, Y
Earnshaw, WC
Mochizuki, Y
Dohmae, N
Todokoro, K
机构
[1] RIKEN, Inst Phys & Chem Res, Cell Fate Signaling Res Unit, Wako, Saitama 3510198, Japan
[2] Univ Edinburgh, Inst Cell & Mol Biol, Wellcome Trust Ctr Cell Biol, Edinburgh, Midlothian, Scotland
[3] RIKEN, Inst Phys & Chem Res, Biomol Characterizat Div, Wako, Saitama, Japan
基金
英国惠康基金;
关键词
central spindle; KIF4; midzone; PRC1;
D O I
10.1038/sj.emboj.7600347
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A number of proteins accumulate in the anaphase spindle midzone, but the interaction and precise role of these proteins in midzone organization remain obscure. Here, we found that the microtubule-bundling protein PRC1 bound separately to the three motor proteins, KIF4, MKLP1 and CENP-E, but not to the chromosomal passenger proteins. In KIF4-deficient cells, the central spindle was disorganized, and all midzone-associated proteins including PRC1 failed to concentrate at the midline, instead being dispersed along the loosened microtubule bundles of the central spindle. This suggests that KIF4 is essential for the organization of central spindles and for midzone formation. In PRC1-deficient cells, no midzone was formed, KIF4 and CENP-E did not localize to the disconnected half-spindle, and MKLP1 and chromosomal passenger proteins localized to discrete subdomains near microtubule plus ends in the half-spindle. Thus, PRC1 is required for interaction of the two half-spindles and for localization of KIF4 and CENP-E. These results suggest that KIF4 and its binding partner PRC1 play essential roles in the organization of central spindles and midzone formation.
引用
收藏
页码:3237 / 3248
页数:12
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