The structure of the keratan sulphate chains attached to fibromodulin from human articular cartilage

被引:19
作者
Lauder, RM [1 ]
Huckerby, TN [1 ]
Nieduszynski, IA [1 ]
机构
[1] UNIV LANCASTER,CTR POLYMER,SCH PHYS & CHEM,LANCASTER LA1 4YQ,ENGLAND
关键词
fibromodulin; small proteoglycan; keratan sulphate; glycosaminoglycan; sulphation; keratanase II; human;
D O I
10.1023/A:1018552913584
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The small keratan sulphate proteoglycan, fibromodulin, has been isolated from pooled human articular cartilage. The main chain repeat region and the chain caps from the attached N-linked keratan sulphate chains have been fragmented by keratanase II digestion, and the oligosaccharides generated have been reduced and isolated. Their structures and abundance have been determined by high pH anion-exchange chromatography. These regions of the keratan sulphate from human articular cartilage fibromodulin have been found to have the following general structure: [GRAPHICS] Significantly, both alpha(2-6)- and alpha(2-3)-linked N-acetyl-neuraminic acid have been found in the capping oligosaccharides. Fucose, which is alpha(1-3)-linked as a branch to N-acetylglucosamine, has also been found along the length of the repeat region and in the capping region. The chains, which have been found to be very highly sulphated, are short; the length of the repeat region and chain caps is ca. nine disaccharides. These data demonstrate that the structure of the N-linked keratan sulphate chains of human articular cartilage fibromodulin is similar, in general, to articular cartilage derived O-linked keratan sulphate chains. Further, the general structure of the keratan sulphate chains attached to human articular cartilage fibromodulin has been found to be generally similar to that of both bovine and equine articular cartilage fibromodulin.
引用
收藏
页码:651 / 660
页数:10
相关论文
共 35 条
[1]  
ANTONSSON P, 1991, J BIOL CHEM, V266, P16859
[2]   STRUCTURE AND DEDUCED AMINO-ACID-SEQUENCE OF THE HUMAN FIBROMODULIN GENE [J].
ANTONSSON, P ;
HEINEGARD, D ;
OLDBERG, A .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1174 (02) :204-206
[3]  
BHAVANANDAN VP, 1968, J BIOL CHEM, V243, P1052
[4]  
BRAY BA, 1967, J BIOL CHEM, V242, P3373
[5]   DEGRADATION OF ARTICULAR-CARTILAGE KERATAN SULFATES USING HYDRAZINOLYSIS AND NITROUS-ACID - ENVIRONMENT OF FUCOSE RESIDUES [J].
BROWN, GM ;
HUCKERBY, TN ;
MORRIS, HG ;
NIEDUSZYNSKI, IA .
BIOCHEMICAL JOURNAL, 1992, 286 :235-241
[6]   OLIGOSACCHARIDES DERIVED FROM BOVINE ARTICULAR-CARTILAGE KERATAN SULFATES AFTER KERATANASE-II DIGESTION - IMPLICATIONS FOR KERATAN SULFATE STRUCTURAL FINGERPRINTING [J].
BROWN, GM ;
HUCKERBY, TN ;
MORRIS, HG ;
ABRAM, BL ;
NIEDUSZYNSKI, IA .
BIOCHEMISTRY, 1994, 33 (16) :4836-4846
[7]   SKELETAL KERATAN SULFATE STRUCTURAL-ANALYSIS USING KERATANASE-II DIGESTION FOLLOWED BY HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY [J].
BROWN, GM ;
NIEDUSZYNSKI, IA ;
MORRIS, HG ;
ABRAM, BL ;
HUCKERBY, TN ;
BLOCK, JA .
GLYCOBIOLOGY, 1995, 5 (03) :311-317
[8]   Characterization of a non-reducing terminal fragment from bovine articular cartilage keratan sulphates containing alpha(2-3)-linked sialic acid and alpha(1-3)-linked fucose [J].
Brown, GM ;
Huckerby, TN ;
Abram, BL ;
Nieduszynski, IA .
BIOCHEMICAL JOURNAL, 1996, 319 :137-141
[9]   SKELETAL KERATAN SULFATE CHAINS ISOLATED FROM BOVINE INTERVERTEBRAL-DISK MAY TERMINATE IN ALPHA(2-]6)-LINKED N-ACETYLNEURAMINIC ACID [J].
DICKENSON, JM ;
HUCKERBY, TN ;
NIEDUSZYNSKI, IA .
BIOCHEMICAL JOURNAL, 1992, 282 :267-271
[10]   A NONREDUCING TERMINAL FRAGMENT FROM TRACHEAL CARTILAGE KERATAN SULFATE CHAINS CONTAINS ALPHA-(2-3)-LINKED N-ACETYLNEURAMINIC ACID [J].
DICKENSON, JM ;
HUCKERBY, TN ;
NIEDUSZYNSKI, IA .
BIOCHEMICAL JOURNAL, 1991, 278 :779-785