Molecular differentiation of alfalfa weevil strains (Coleoptera: Curculionidae)

被引：23

作者：

Erney, SJ ^{[1
]}

Pruess, KP ^{[1
]}

Danielson, SD ^{[1
]}

Powers, TO ^{[1
]}

机构：

[1] UNIV NEBRASKA, DEPT ENTOMOL, LINCOLN, NE 68583 USA

来源：

ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA | 1996年 / 89卷 / 06期

关键词：

Hypera postica; alfalfa weevil strains; mtDNA; PCR-RFLP; DNA sequence;

D O I：

10.1093/aesa/89.6.804

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Mitochondrial DNA was amplified and sequenced from eastern, western, and Egyptian strains of alfalfa weevil, Hypera postica (Cyllenhal). Eastern and Egyptian weevils differed at only 2 nucleotide sites in 1,031 base pairs sequenced; western weevils differed by 5% sequence divergence. Three restriction sites were identified which separated eastern and western haplotypes. No intrastrain polymorphism was detected in 150 weevils from Nebraska. Collections from Lincoln in eastern Nebraska and Scottsbluff in western Nebraska were fixed for the eastern and western haplotypes, respectively. Eastern and western haplotypes were found together in the same fields in a broad overlap region in central Nebraska.

引用

页码：804 / 811

页数：8

共 22 条

[1] MATING PREFERENCE OF EASTERN AND WESTERN UNITED-STATES STRAINS OF ALFALFA-WEEVIL COLEOPTERA-CURCULIONIDAE [J].