Specificity of different anti-myosin heavy chain antibodies in bovine muscle

被引：26

作者：

Duris, MP ^{[1
]}

Picard, B ^{[1
]}

Geay, Y ^{[1
]}

机构：

[1] INRA Clermont Ferrand Theix, Equipe Croissance & Metab Muscle, Unite Rech Herbivores, F-63122 St Genes Champanelle, France

来源：

MEAT SCIENCE | 2000年 / 55卷 / 01期

关键词：

antibody; myosin heavy chain; bovine and muscle;

D O I：

10.1016/S0309-1740(99)00127-8

中图分类号：

TS2 [食品工业];

学科分类号：

0832 [食品科学与工程];

摘要：

Muscle fibre types play an important role in bovine meat tenderness, and conditions the speed of ageing. Two generations of myotubes appear during foetal life. Their differentiation results in several types of fibres differing in their contractile and metabolic properties, namely type I fibres and type II fibres (IIA and IIB). Fibre types were identified using antibodies by immunohistochemistry and western-blotting techniques. The aim was to test different antibodies on foetal and adult muscles by these two techniques. Ten monoclonal antibodies reported to be specific to different myosin heavy chain (MHC) isoforms in other species were tested. Only three antibodies were selected: BF.35, specific for I and IIa MHCs, F1.652 specific for foetal MHC and SC.71 an anti II MHCs (IIa and IIb) antibody. The use of these antibodies will enable a more precise characterisation of muscle fibres and will have applications in the study of bovine muscle ontogenesis. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：67 / 78

页数：12

共 27 条

[1]

3 FAST MYOSIN HEAVY-CHAINS IN ADULT-RAT SKELETAL-MUSCLE [J].

BAR, A ;

PETTE, D .

FEBS LETTERS, 1988, 235 (1-2) :153-155

[2]

BECHET DM, 1991, J BIOL CHEM, V266, P14104

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4]

Comparison of foetal metabolic differentiation in three cattle muscles [J].

Gagnière, H ;

Picard, B ;

Jurie, C ;

Geay, Y .

REPRODUCTION NUTRITION DEVELOPMENT, 1999, 39 (01) :105-112

[5]

GAGNIERE H, 1996, DIFFERENCITION CONTR, P1

[6]

IDENTIFICATION OF A NOVEL TYPE-2 FIBER POPULATION IN MAMMALIAN SKELETAL-MUSCLE BY COMBINED USE OF HISTOCHEMICAL MYOSIN ATPASE AND ANTIMYOSIN MONOCLONAL-ANTIBODIES [J].

GORZA, L .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1990, 38 (02) :257-265

[7]

PATTERNS OF MYOSIN ISOFORMS IN MAMMALIAN SKELETAL-MUSCLE FIBERS [J].

HAMALAINEN, N ;

PETTE, D .

MICROSCOPY RESEARCH AND TECHNIQUE, 1995, 30 (05) :381-389

[8]

THE HISTOCHEMICAL PROFILES OF FAST FIBER TYPE-IIB, TYPE-IID, AND TYPE-IIA IN SKELETAL-MUSCLES OF MOUSE, RAT, AND RABBIT [J].

HAMALAINEN, N ;

PETTE, D .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1993, 41 (05) :733-743

[9]

CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10]

Lefaucheur L, 1998, J ANIM SCI, V76, P1584

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