Hyperpolarized 1H NMR Employing Low γ Nucleus for Spin Polarization Storage

Here, we demonstrate the utility of low gamma nuclei for spin storage of hyperpolarization followed by proton detection, which theoretically can provide up to similar to (gamma[1H]/gamma[X])(2) gain in sensitivity in hyperpolarized biomedical MR. This is exemplified by hyperpolarized 1-C-13 sites of 2,2,3,3-tetrafluoropropyl 1-C-13-propionate-d(3) (TFPP), C-13 T-1 = 67 s in D2O, and 1-C-13-succinate-d(2), C-13 T-1 = 105 s in D2O, pH 11, using PASADENA. In a representative example, the spin polarization was stored on C-13 for 24 and 70 s, respectively, white the samples were transferred from a tow magnetic field polarizer operating at 1.76 mT to a 4.7 T animal MR scanner. Following sample delivery, the refocused INEPT pulse sequence was used to transfer spin polarization from C-13 to protons with an efficiency of 50% for TFPP and 41% for 1-C-13-succinate-d(2) increasing the overall NMR sensitivity by a factor of 7.9 and 6.5, respectively. The low gamma nuclei exemplified here by C-13 with a T-1 of tens of seconds acts as an efficient spin polarization storage, while J-coupled protons are better for NMR detection.