Bacterial diversity and community structure in the East China Sea by 454 sequencing of the 16S rRNA gene

被引：47

作者：

Dong Yi ^{[1
,2
]}

Zhao Yuan ^{[1
]}

Zhang Wenyan ^{[1
]}

Li Yan ^{[3
]}

Zhou Feng ^{[4
]}

Liu Chenggang ^{[5
]}

Wu Ying ^{[6
]}

Liu Sumei ^{[7
]}

Zhang Wuchang ^{[1
]}

Xiao Tian ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Shandong Acad Sci, Energy Res Inst, Jinan 250014, Peoples R China

[4] State Ocean Adm, Inst Oceanog 2, State Key Lab Satellite Ocean Environm Dynam, Hangzhou 310012, Zhejiang, Peoples R China

[5] State Ocean Adm, Inst Oceanog 2, Lab Marine Ecosyst & Biogeochem, Hangzhou 310012, Zhejiang, Peoples R China

[6] E China Normal Univ, State Key Lab Estuarine & Coastal Res, Shanghai 200062, Peoples R China

[7] Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, Qingdao 266100, Peoples R China

来源：

CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY | 2014年 / 32卷 / 03期

基金：

中国国家自然科学基金;

关键词：

bacterial diversity; community structure; 454; sequencing; water masses; canonical correspondence analysis; East China Sea; DEEP-SEA; SP-NOV; PICOPLANKTON DISTRIBUTION; MICROBIAL COMMUNITIES; CLASS FLAVOBACTERIA; PELAGIC BACTERIA; MARINE SEDIMENT; COASTAL WATERS; SURFACE-WATER; BALTIC SEA;

D O I：

10.1007/s00343-014-3215-2

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

The 454 sequencing method was used to detect bacterial diversity and community structure in the East China Sea. Overall, 149 067 optimized reads with an average length of 454 nucleotides were obtained from 17 seawater samples and five sediment samples sourced in May 2011. A total of 22 phyla, 34 classes, 74 orders, 146 families, and 333 genera were identified in this study. Some of them were detected for the first time from the East China Sea. The estimated richness and diversity indices were both higher in the sediment samples compared with in the seawater samples. All the samples were divided by their diversity indices into four regions. Similarity analysis showed that the seawater samples could be classified into six groups. The groups differed from each other and had unique community structure characteristics. It was found that different water masses in the sampling areas may have had some influence on the bacterial community structure. A canonical correspondence analysis revealed that seawater samples in different areas and at different depths were affected by different environmental parameters. This study will lay the foundation for future research on microbiology in the East China Sea.

引用

页码：527 / 541

页数：15

共 104 条

[1]

Colonization and community dynamics of class Flavobacteria on diatom detritus in experimental mesocosms based on Southern Ocean seawater [J].