Biochemical and immunochemical evidences for the presence of lipoxygenase in plant mitochondria

被引:14
作者
Braidot, E
Petrussa, E
Micolini, S
Tubaro, F
Vianello, A
Macrì, F
机构
[1] Univ Udine, Plant Biol Sect, Dept Biol & Agroind Econ, I-33100 Udine, Italy
[2] Univ Udine, Dept Chem Sci & Technol, I-33100 Udine, Italy
关键词
lipoxygenase; mitochondria; Pisum sativum L;
D O I
10.1093/jxb/erh197
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In this paper, both biochemical and immunochemical evidence for the presence of lipoxygenase (LOX) in plant mitochondria is presented. Highly purified pea (Pisum sativum L., cv. Alaska) mitochondria show LOX activity, evaluated as conjugated diene formation, oxygen consumption, and hydroperoxide formation. Both 9- and 13-hydroperoxy-octadecadienoic acids are produced by the oxidation of linoleic acid. LOX activity is particularly evident in swollen mitochondria; it is inhibited by nordihydroguaiaretic acid, a pea anti-LOX B antibody, and has two pH optima (6.0 and 7.5). A mitochondrial protein of similar to97 kDa cross-reacts with a pea seed anti-LOX B antibody. This reaction is detectable in both soluble (matrix fraction) and membrane-bound (submitochondrial particles) proteins. Considering that pea mitochondria were extracted from actively growing stems that were differentiating tube elements, it is suggested that the presence of LOX in these organelles may be related to their degradation linked to xylem differentiation.
引用
收藏
页码:1655 / 1662
页数:8
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