Monitoring surface-assisted biomolecular assembly by means of evanescent-field-coupled waveguide-mode nanobiosensors

被引：15

作者：

Gopinath, Subash C. B. ^{[1
]}

Awazu, Koichi ^{[1
]}

Fujimaki, Makoto ^{[1
]}

Sugimoto, Katsuaki ^{[2
]}

Ohki, Yoshimichi ^{[2
]}

Komatsubara, Tetsuro ^{[3
]}

Tominaga, Junji ^{[1
]}

Kumar, Penmetcha K. R. ^{[4
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, CAN FOR, Tsukuba, Ibaraki 3058562, Japan

[2] Waseda Univ, Tokyo 1698555, Japan

[3] Univ Tsukuba, Tsukuba, Ibaraki 3058577, Japan

[4] AIST, Inst Biol Resources & Funct, Tsukuba, Ibaraki 3058566, Japan

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2009年 / 394卷 / 02期

关键词：

Biomolecular assembly; Nanobiosensor; Evanescent-field-coupled waveguide; SiO2; Aptamer; PROTEIN-PROTEIN INTERACTIONS; IX-BINDING PROTEIN; RNA APTAMER; BLOOD-COAGULATION; SENSITIVITY; SPECTROSCOPY; SENSORS; FILMS; VENOM;

D O I：

10.1007/s00216-009-2721-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Biological self-assembly is a natural process that involves various biomolecules, and finding the missing partner in these interactions is crucial for a specific biological function. Previously, we showed that evanescent-field-coupled waveguide-mode sensor in conjunction with a SiO2 waveguide, the surfaces which contain cylindrical nanometric holes produced by atomic bombardment, allowed us to detect efficiently the biomolecular interactions. In the present studies, we showed that the assembly of biomolecules can be monitored using the evanescent-field-coupled waveguide-mode biosensor and thus provide a methodology in monitoring assembly process in macromolecular machines while they are assembling.

引用

页码：481 / 488

页数：8

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