Proline antagonizes GABA-induced quenching of quorum-sensing in Agrobacterium tumefaciens

被引：99

作者：

Haudecoeur, E. ^{[1
]}

Planamentea, S. ^{[1
,2
]}

Cirou, A. ^{[1
]}

Tannieres, M. ^{[1
]}

Shelp, B. J. ^{[3
]}

Morera, S. ^{[2
]}

Faure, D. ^{[1
]}

机构：

[1] CNRS, Inst Sci Vegetal, F-91198 Gif Sur Yvette, France

[2] CNRS, Lab Enzymol & Biochim Struct, F-91198 Gif Sur Yvette, France

[3] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 34期

关键词：

plant defense; virulence; plant tumor; lactonase; SIGNAL SALICYLIC-ACID; X-RAY STRUCTURES; SUBSTRATE-SPECIFICITY; BINDING-PROTEIN; POTENTIAL ROLE; ARABIDOPSIS; GENE; EXPRESSION; RECEPTOR; TRANSFORMATION;

D O I：

10.1073/pnas.0808005106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plants accumulate free L-proline (Pro) in response to abiotic stresses (drought and salinity) and presence of bacterial pathogens, including the tumor-inducing bacterium Agrobacterium tumefaciens. However, the function of Pro accumulation in host-pathogen interaction is still unclear. Here, we demonstrated that Pro antagonizes plant GABA-defense in the A. tumefaciens C58-induced tumor by interfering with the import of GABA and consequently the GABA-induced degradation of the bacterial quorum-sensing signal, 3-oxo-octanoylhomoserine lactone. We identified a bacterial receptor Atu2422, which is implicated in the uptake of GABA and Pro, suggesting that Pro acts as a natural antagonist of GABA-signaling. The Atu2422 amino acid sequence contains a Venus flytrap domain that is required for trapping GABA in human GABA(B) receptors. A constructed atu2422 mutant was more virulent than the wild type bacterium; moreover, transgenic plants with a low level of Pro exhibited less severe tumor symptoms than did their wild-type parents, revealing a crucial role for Venus flytrap GABA-receptor and relative abundance of GABA and Pro in host-pathogen interaction.

引用

页码：14587 / 14592

页数：6

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