Decreased peroxisome proliferator-activated receptor gamma gene expression is correlated with poor prognosis in patients with esophageal cancer

被引：71

作者：

Terashita, Y ^{[1
]}

Sasaki, H ^{[1
]}

Haruki, N ^{[1
]}

Nishiwaki, T ^{[1
]}

Ishiguro, H ^{[1
]}

Shibata, Y ^{[1
]}

Kudo, J ^{[1
]}

Konishi, S ^{[1
]}

Kato, J ^{[1
]}

Koyama, H ^{[1
]}

Kimura, M ^{[1
]}

Sato, A ^{[1
]}

Shinoda, N ^{[1
]}

Kuwabara, Y ^{[1
]}

Fujii, Y ^{[1
]}

机构：

[1] Nagoya City Univ, Sch Med, Dept Surg 2, Mizuho Ku, Nagoya, Aichi 4678601, Japan

来源：

JAPANESE JOURNAL OF CLINICAL ONCOLOGY | 2002年 / 32卷 / 07期

关键词：

PPAR gamma; esophageal cancer; prognosis; LightCycler; immunohistochemistry;

D O I：

10.1093/jjco/hyf056

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Peroxisome proliferator-activated receptor gamma (PPARgamma) induces apoptosis by ligand stimulation in various tumor cell lines. In esophageal cancer cell lines, PPARgamma activation also has suppressed the proliferation. Methods: In 55 primary esophageal squamous cell carcinomas (ESCCs) we examined the correlation between the expression of PPARgamma mRNA with prognosis of esophageal cancer patients. The expression of PPARgamma mRNA was quantified by real-time reverse transcription polymerase chain reaction using LightCycler. Immunohistochemistry was used to study the expression of PPARgamma protein. Results: The expression of PPARgamma mRNA was significantly decreased in esophageal cancer cells compared with normal esophageal mucosa (P = 0.0084). Among the clinical factors, PPARgamma mRNA expression was lower in the tumors with extensive lymph node metastasis (n4) than those with less extensive lymph node metastasis (n0-3) (P = 0.0059). Patients with low PPARgamma mRNA expression had significantly shorter postoperative survival time than those with high PPARgamma mRNA expression (P = 0.0191). In immunohistochemistry, PPARgamma protein was expressed in the nuclei of cells in some cases and expressed in the nuclei and cytoplasm in others. The expression of PPARgamma protein is decreased in esophageal cancer tissue compared with normal esophageal squamous epithelium. However, we could not deduce the apparent relation for the expression between PPARgamma mRNA and PPARgamma proteins in immunohistochemistry (P = 0.284). Conclusions: In esophageal cancer tissues, the expression of PPARgamma was decreased compared with normal esophageal epithelium. The mRNA expression level of PPARgamma may be a marker of prognosis after operation in esophageal cancer patients.

引用

页码：238 / 243

页数：6

共 28 条

[1] Activation of PPARγ leads to inhibition of anchorage-independent growth of human colorectal cancer cells [J].

Brockman, JA ;

Gupta, RA ;

DuBois, RN .

GASTROENTEROLOGY, 1998, 115 (05) :1049-1055

[2]

Chang TH, 2000, CANCER RES, V60, P1129

[3] Induction of solid tumor differentiation by the peroxisome proliferator-activated receptor-γ ligand troglitazone in patients with liposarcoma [J].

Demetri, GD ;

Fletcher, CDM ;

Mueller, E ;

Sarraf, P ;

Naujoks, R ;

Campbell, N ;

Spiegelman, BM ;

Singer, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (07) :3951-3956

[4] The nuclear eicosanoid receptor, PPARγ, is aberrantly expressed in colonic cancers [J].

DuBois, RN ;

Gupta, R ;

Brockman, J ;

Reddy, BS ;

Krakow, SL ;

Lazar, MA .

CARCINOGENESIS, 1998, 19 (01) :49-53

[5]

Elnemr A, 2000, INT J ONCOL, V17, P1157

[6]

Guan You-Fei, 1999, Neoplasia (New York), V1, P330, DOI 10.1038/sj.neo.7900050

[7]

Han SW, 2001, CLIN CANCER RES, V7, P98

[8]

Imamura M, 2001, J AM COLL SURGEONS, V192, P708

[9] Prognostic value of p27Kip1 and cyclinD1 expression in esophageal cancer [J].

Itami, A ;

Shimada, Y ;

Watanabe, G ;

Imamura, M .

ONCOLOGY, 1999, 57 (04) :311-317

[10]

*JAP SOC ES DIS, 1999, GUID CLIN PATH STUD

← 1 2 3 →