Localization of Haemophilus ducreyi at the pustular stage of disease in the human model of infection

被引:47
作者
Bauer, ME
Spinola, SM
机构
[1] Indiana Univ, Sch Med, Dept Med, Indianapolis, IN 46202 USA
[2] Indiana Univ, Sch Med, Dept Microbiol & Immunol, Indianapolis, IN 46202 USA
[3] Indiana Univ, Sch Med, Dept Pathol & Lab Med, Indianapolis, IN 46202 USA
关键词
D O I
10.1128/IAI.68.4.2309-2314.2000
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
To localize Haemophilus ducreyi in vivo, human subjects were experimentally infected with H. ducreyi until they developed a painful pustule or for 14 days. Lesions were biopsied, and biopsy samples were fixed in 4% paraformaldehyde, and cryosectioned, Sections were stained with polyclonal anti-H. ducreyi antiserum or H. ducreyi-specific monoclonal antibodies (MAbs) and fluorescently tagged secondary antibodies and examined by confocal microscopy. We identified H. ducreyi in 16 of 18 pustules but did not detect bacteria in the one papule examined. H. ducreyi was observed as individual cells and in clumps or chains. Staining with MAbs 2D8, 5C9, 3B9, 2C7, and 9D12 demonstrated that H. ducreyi expresses the major pilus subunit, FtpA, the 28-kDa outer membrane protein Hlp, the 18-kDa outer membrane protein PAL, and the major outer membrane protein (MOMP) or OmpA2 in vivo. By dual staining with polyclonal anti-II. ducreyi antiserum and MAbs that recognize human skin components, we observed bacteria within the neutrophilic infiltrates of all positively staining pustules and in the dermis of 10 of 16 pustules, We were unable to detect bacteria associated with keratinocytes in the samples examined. The data suggest that II, ducreyi is found primarily in association with neutrophils and in the dermis at the pustular stage of disease in the human model of infection.
引用
收藏
页码:2309 / 2314
页数:6
相关论文
共 31 条
[1]   BINDING OF HAEMOPHILUS-DUCREYI TO EXTRACELLULAR-MATRIX PROTEINS [J].
ABECK, D ;
JOHNSON, AP ;
MENSING, H .
MICROBIAL PATHOGENESIS, 1992, 13 (01) :81-84
[2]   Standardization of the experimental model of Haemophilus ducreyi infection in human subjects [J].
Al-Tawfiq, JA ;
Thornton, AC ;
Katz, BP ;
Fortney, KR ;
Todd, KD ;
Hood, AF ;
Spinola, SM .
JOURNAL OF INFECTIOUS DISEASES, 1998, 178 (06) :1684-1687
[3]   Cumulative experience with Haemophilus ducreyi 35000 in the human model of experimental infection [J].
Al-Tawfiq, JA ;
Harezlak, J ;
Katz, BP ;
Spinola, SM .
SEXUALLY TRANSMITTED DISEASES, 2000, 27 (02) :111-114
[4]   HAEMOPHILUS-DUCREYI ADHERES TO BUT DOES NOT INVADE CULTURED HUMAN FORESKIN CELLS [J].
ALFA, MJ ;
DEGAGNE, P ;
HOLLYER, T .
INFECTION AND IMMUNITY, 1993, 61 (05) :1735-1742
[5]  
ALTAWIFIQ JA, IN PRESS J INFECT DI
[6]   Binding of Haemophilus ducreyi to extracellular matrix proteins [J].
Bauer, ME ;
Spinola, SM .
INFECTION AND IMMUNITY, 1999, 67 (05) :2649-2652
[7]   Fine tangled pili expressed by Haemophilus ducreyi are a novel class of pili [J].
Brentjens, RJ ;
Ketterer, M ;
Apicella, MA ;
Spinola, SM .
JOURNAL OF BACTERIOLOGY, 1996, 178 (03) :808-816
[8]   HAEMOPHILUS-DUCREYI ADHERES TO HUMAN KERATINOCYTES [J].
BRENTJENS, RJ ;
SPINOLA, SM ;
CAMPAGNARI, AA .
MICROBIAL PATHOGENESIS, 1994, 16 (03) :243-247
[9]   From epidemiological synergy to public health policy and practice: the contribution of other sexually transmitted diseases to sexual transmission of HIV infection [J].
Fleming, DT ;
Wasserheit, JN .
SEXUALLY TRANSMITTED INFECTIONS, 1999, 75 (01) :3-17
[10]   HISTOLOGICAL ASPECTS OF SEXUALLY-TRANSMITTED GENITAL LESIONS [J].
FREINKEL, AL .
HISTOPATHOLOGY, 1987, 11 (08) :819-831