Integrin-linked kinase (ILK) regulation of the cell viability in PTEN mutant glioblastoma and in vitro inhibition by the specific COX-2 inhibitor NS-398

被引：29

作者：

Obara, S

Nakata, M

Takeshima, H

Katagiri, H

Asano, T

Oka, Y

Maruyama, I

Kuratsu, J

机构：

[1] Kagoshima Univ, Fac Med, Dept Lab & Mol Med, Kagoshima 8908520, Japan

[2] Jichi Med Sch, Dept Physiol, Minami Kawachi, Tochigi 3290498, Japan

[3] Tohoku Univ, Grad Sch Med, Dept Internal Med, Div Mol Metab & Diabet, Sendai, Miyagi 9808574, Japan

[4] Univ Tokyo, Fac Med, Dept Internal Med, Bunkyo Ku, Tokyo 1138655, Japan

[5] Kagoshima Univ, Fac Med, Dept Neurosurg, Kagoshima 8908520, Japan

来源：

CANCER LETTERS | 2004年 / 208卷 / 01期

关键词：

glioblastoma; ILK; PTEN; PKB/Akt-Serine; 473; NS-398;

D O I：

10.1016/j.canlet.2003.11.020

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We report the increased activity and expression of the ILK protein in human glioblastomas and demonstrate that ILK activity is regulated by PTEN. The transfection of wild type-PTEN into the glioblastoma cell line U-251 MG altered the localization of ILK in the cell membrane: transfection with PTEN down-regulated PKB/Akt-Ser-473 phosphorylation via the inhibition of ILK-signaling. Our results suggest that ILK is critical for the PTEN-sensitive regulation of PKB/Akt-dependent cell survival. The selective COX-2 inhibitor NS-398 was found capable of down-regulating ILK and PKB/Akt phosphorylation. Our data indicate that inhibition of ILK signaling may be beneficial in the treatment of PTEN-deficient glioblastoma. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：115 / 122

页数：8

共 39 条

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