Elevated level of microRNA-21 in the serum of patients with colorectal cancer

被引:58
作者
Basati, Gholam [1 ]
Razavi, Amirnader Emami [2 ]
Abdi, Soheila [3 ]
Mirzaei, Asad [4 ]
机构
[1] Ilam Univ Med Sci, Fac Allied Med Sci, Dept Clin Biochem, Ilam, Iran
[2] Univ Tehran Med Sci, Inst Canc, Iran Natl Tumor Bank, Tehran, Iran
[3] Islamic Azad Univ, Safadasht Branch, Dept Phys, Tehran, Iran
[4] Ilam Univ Med Sci, Dept Parasitol, Fac Med, Ilam, Iran
关键词
Serum miR-21; Colorectal adenocarcinoma; Clinical staging; qRT-PCR; EXPRESSION PROFILES; MIR-21; BIOMARKER; PROGNOSIS;
D O I
10.1007/s12032-014-0205-3
中图分类号
R73 [肿瘤学];
学科分类号
100214 [肿瘤学];
摘要
In patients with colorectal cancer, circulating micro RNA-21 (miR-21) is overexpressed and may act as a potential diagnostic and prognostic biomarker. In the present study, serum miR-21 level was determined in patients with colorectal cancer and control subjects in an attempt to explore its potential clinical diagnostic and prognostic value. Serum levels of miR-21 were measured in 40 patients with colorectal adenocarcinoma and 40 control subjects using a quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) assay. Serum miR-21 levels were compared in the colorectal cancer patients and control subjects. Furthermore, the association between serum miR-21 level and the clinical stages of tumors was also examined in the patients. Serum miR-21 level was significantly elevated in colorectal adenocarcinoma patients relative to control subjects (P = 0.0001), and it was revealed as a potential diagnostic biomarker for differentiating the patients from control subjects. Increased levels of serum miR-21 were associated with clinical stages of tumors in the patients (P = 0.01). These results indicated that serum miR-21 levels could serve as a reliable diagnostic and prognostic biomarker for colorectal adenocarcinoma.
引用
收藏
页码:1 / 5
页数:5
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