Authentication of meats from quail (Coturnix coturnix), pheasant (Phasianus colchicus), partridge (Alectoris spp.), and guinea fowl (Numida meleagris) using polymerase chain reaction targeting specific sequences from the mitochondrial 12S rRNA gene

被引：24

作者：

Rojas, Maria ^{[1
]}

Gonzalez, Isabel ^{[1
]}

Fajardo, Violeta ^{[1
]}

Martin, Irene ^{[1
]}

Hernandez, Pablo E. ^{[1
]}

Garcia, Teresa ^{[1
]}

Martin, Rosario ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

来源：

FOOD CONTROL | 2009年 / 20卷 / 10期

关键词：

Game birds; 12S rRNA gene; Species-specific PCR; FRAGMENT LENGTH POLYMORPHISM; CHAMOIS RUPICAPRA-RUPICAPRA; DEER CAPREOLUS-CAPREOLUS; IBEX CAPRA-PYRENAICA; MOUFLON OVIS-AMMON; PCR-RFLP; CERVUS-ELAPHUS; DAMA-DAMA; IDENTIFICATION; DNA;

D O I：

10.1016/j.foodcont.2008.12.011

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Polymerase chain reaction (PCR) based on oligonucleotide primers targeting the mitochondrial 12S rRNA gene has been applied to the specific identification of meats from quail (Coturnix coturnix), pheasant (Phasianus colchicus), partridge (Alectoris spp.), and guinea fowl (Numida meleagris). The use of specific primers pairs for quail, pheasant, partridge and guinea fowl allowed the selective amplification of the desired avian sequences. The specificity of each primer pair was verified by PCR analysis of DNA from meats of various game and domestic bird and mammalian species. The assay can be useful for the accurate identification of meats from game bird species, avoiding mislabelling or fraudulent species substitution in meat products. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：896 / 902

页数：7

共 23 条

[1] Identification of species in animal feedstuffs by polymerase chain reaction-restriction fragment length polymorphism analysis of mitochondrial DNA [J].

Bellagamba, F ;

Moretti, VM ;

Comincini, S ;

Valfrè, F .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (08) :3775-3781

[2] Development of a DNA-based assay for species identification in meat and bone meal [J].

Colgan, S ;

O'Brien, L ;

Maher, M ;

Shilton, N ;

McDonnell, K ;

Ward, S .

FOOD RESEARCH INTERNATIONAL, 2001, 34 (05) :409-414

[3] Identification of the goose species (Anser anser) in Italian "Mortara" salami by DNA sequencing and a Polymerase Chain Reaction with an original primer pair [J].

Colombo, F ;

Marchisio, E ;

Pizzini, A ;

Cantoni, C .

MEAT SCIENCE, 2002, 61 (03) :291-294

[4] A multiplex PCR assay for the identification of animal species in feedstuffs [J].

Dalmasso, A ;

Fontanella, E ;

Piatti, P ;

Civera, T ;

Rosati, S ;

Bottero, MT .

MOLECULAR AND CELLULAR PROBES, 2004, 18 (02) :81-87

[5] Duplex polymerase chain reaction for detection of pork meat in horse meat fresh sausages from Italian retail sources [J].

Di Pinto, A ;

Forte, VT ;

Conversano, MC ;

Tantillo, GM .

FOOD CONTROL, 2005, 16 (05) :391-394

[6] PCR identification of meats from chamois (Rupicapra rupicapra), pyrenean ibex (Capra pyrenaica), and mouflon (Ovis ammon) targeting specific sequences from the mitochondrial D-loop region [J].

Fajardo, V. ;

Gonzalez, I. ;

Lopez-Calleja, I. ;

Martin, I. ;

Rojas, M. ;

Garcia, T. ;

Hernandez, P. E. ;

Martin, Rosario .

MEAT SCIENCE, 2007, 76 (04) :644-652

[7] Identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama), and roe deer (Capreolus capreolus) using polymerase chain reaction targeting specific sequences from the mitochondrial 12S rRNA gene [J].

Fajardo, V. ;

Gonzalez, I. ;

Lopez-Calleja, I. ;

Martin, I. ;

Rojas, M. ;

Hernandez, P. E. ;

Garcia, T. ;

Martin, Rosario .

MEAT SCIENCE, 2007, 76 (02) :234-240

[8] PCR-RFLP authentication of meats from red deer (Cervus elaphus), fallow deer (Dama dama), roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries), and goat (Capra hircus) [J].

Fajardo, V ;

González, I ;

López-Calleja, I ;

Martín, I ;

Hernández, PE ;

García, T ;

Martín, R .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (04) :1144-1150

[9]

Fajardo V, 2007, J AOAC INT, V90, P179

[10] Polymerase chain reaction-restriction fragment length polymorphism of mitochondrial 12S rRNA gene: A simple method for identification of poultry meat species [J].

Girish, P. S. ;

Anjaneyulu, A. S. R. ;

Viswas, K. N. ;

Santhosh, F. H. ;

Bhilegaonkar, K. N. ;

Agarwal, R. K. ;

Kondaiah, N. ;

Nagappa, K. .

VETERINARY RESEARCH COMMUNICATIONS, 2007, 31 (04) :447-455

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