Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP-dependent protein kinase II

A yeast two-hybrid screen revealed that regulatory subunits (RII) of PKAII bind the Yotiao protein. Yotiao interacts with the NR1 subunit of the NMDA receptor, A purified C-terminal fragment of Yotiao binds PKAII, via an RII binding site constituted by amino acid residues 1452-1469, with a dissociation constant (K-d) between 50 and 90 nM in vitro, A stable complex composed of Yotiao, RII and NRL was immunoprecipitated from whole rat brain extracts, Immunostaining analysis disclosed that Yotiao, RII beta and NR1 colocalize in striatal and cerebellar neurons, Go-assembly of Yotiao/PKAII complexes with NR1 subunits may promote cAMP-dependent modulation of NMDA receptor activity at synapses, thereby influencing brain development and synaptic plasticity, (C) 1999 Federation of European Biochemical Societies.