Detection of granulocytic Ehrlichia species DNA by PCR in persistently infected dogs

被引:81
作者
Egenvall, A [1 ]
Lilliehöök, I
Bjöersdorff, A
Engvall, EO
Karlstam, E
Artursson, K
Heldtander, M
Gunnarsson, A
机构
[1] Swedish Univ Agr Sci, Dept Small Anim Clin Sci, Uppsala, Sweden
[2] Swedish Univ Agr Sci, Dept Clin Chem, Uppsala, Sweden
[3] Swedish Univ Agr Sci, Dept Pathol, Uppsala, Sweden
[4] Kalmar Cty Hosp, Dept Clin Microbiol, Kalmar, Sweden
[5] Natl Vet Inst, Dept Bacteriol, S-75007 Uppsala, Sweden
关键词
D O I
10.1136/vr.146.7.186
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Three female beagle dogs inoculated with granulocytic Ehrlichia species were monitored for four to six months to determine whether there was evidence that the organisms persisted. The dogs were inoculated intravenously with blood containing an Ehrlichia species closely related to Ehrlichia equi and Ehrlichia phagocytophila. and identical to the human granulocytic ehrlichiosis agent with respect to its 16S rRNA gene sequence. The clinical signs were evaluated, and blood samples were collected for haematology, serum biochemistry and serology. Ehrlichial inclusions in the blood were monitored by microscopy, and ehrlichial DNA was detected by the polymerase chain reaction (PCR). Two of the dogs were injected with prednisolone on days 54 to 56 and days 152 to 154 after infection, and the other was injected with prednisolone on days 95 to 97 after infection. The dogs were euthanased and examined postmortem. Ehrlichial inclusions were demonstrated in the neutrophils and seroconversion occurred shortly after inoculation. Two of the dogs developed acute disease with rectal temperatures above 39.0 degrees C, after which no further clinical signs were observed. The administration of corticosteroids seemed to facilitate the detection of ehrlichial inclusions. Ehrlichial DNA was detected intermittently by PCR in blood samples from two of the dogs throughout the study. Persistent infection was demonstrated up to five-and-a-half months after inoculation.
引用
收藏
页码:186 / 190
页数:5
相关论文
共 28 条
[1]  
BADYLAK SF, 1981, AM J VET RES, V42, P1310
[2]   Nested polymerase chain reaction for detection of Ehrlichia equi genomic DNA in horses and ticks (Ixodes pacificus) [J].
Barlough, JE ;
Madigan, JE ;
DeRock, E ;
Bigornia, L .
VETERINARY PARASITOLOGY, 1996, 63 (3-4) :319-329
[3]  
BOSSAK ET, 1955, P SOC EXP BIOL MED, V88, P634
[4]   TROPICAL CANINE PANCYTOPENIA - CLINICAL, HEMATOLOGIC, AND SEROLOGIC RESPONSE OF DOGS TO EHRLICHIA-CANIS INFECTION, TETRACYCLINE THERAPY, AND CHALLENGE INOCULATION [J].
BUHLES, WC ;
HUXSOLL, DL ;
RISTIC, M .
JOURNAL OF INFECTIOUS DISEASES, 1974, 130 (04) :357-367
[5]   Experimental infection of the human granulocytic ehrlichiosis agent in horses [J].
Chang, YF ;
Novosel, V ;
Dubovi, E ;
Wong, SJ ;
Chu, FK ;
Chang, CF ;
Del Piero, F ;
Shin, S ;
Lein, DH .
VETERINARY PARASITOLOGY, 1998, 78 (02) :137-145
[6]   IDENTIFICATION OF A GRANULOCYTOTROPIC EHRLICHIA SPECIES AS THE ETIOLOGIC AGENT OF HUMAN-DISEASE [J].
CHEN, SM ;
DUMLER, JS ;
BAKKEN, JS ;
WALKER, DH .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (03) :589-595
[7]   DETECTION OF HUMORAL-ANTIGEN AND ANTIBODY BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY IN HORSES WITH EXPERIMENTALLY INDUCED EHRLICHIA-EQUI INFECTION [J].
CORSTVET, RE ;
GAUNT, SD ;
KARNS, PA ;
MCBRIDE, JW ;
BATTISTINI, RA ;
MAUTERER, LA ;
AUSTIN, FW .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1993, 5 (01) :37-39
[8]   Coinfection with Borrelia burgdorferi and the agent of human granulocytic ehrlichiosis [J].
Duffy, J ;
Pittlekow, MR ;
Kolbert, CP ;
Rutledge, BJ ;
Persing, DH .
LANCET, 1997, 349 (9049) :399-399
[9]   Human granulocytic Ehrlichiosis in Wisconsin and Minnesota: A frequent infection with the potential for persistence [J].
Dumler, JS ;
Bakken, JS .
JOURNAL OF INFECTIOUS DISEASES, 1996, 173 (04) :1027-1030
[10]   PERSISTENT INFECTION WITH EHRLICHIA-CHAFFEENSIS [J].
DUMLER, JS ;
SUTKER, WL ;
WALKER, DH .
CLINICAL INFECTIOUS DISEASES, 1993, 17 (05) :903-905