Phospholipin, a novel heterodimeric phospholipase A2 from Pandinus imperator scorpion venom

被引：46

作者：

Conde, R ^{[1
]}

Zamudio, FZ ^{[1
]}

Becerril, B ^{[1
]}

Possani, LD ^{[1
]}

机构：

[1] Natl Autonomous Univ Mexico, Inst Biotechnol, Dept Mol Recognit & Struct Biol, Cuernavaca 62210, Morelos, Mexico

来源：

FEBS LETTERS | 1999年 / 460卷 / 03期

关键词：

phospholipase A2; amino acid sequence; nucleotide sequence; scorpion venom; Pandinus imperator;

D O I：

10.1016/S0014-5793(99)01392-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The primary structure of a phospholipase A2, with unique structural and functional characteristics, was determined. The large subunit has 108 amino acid residues, linked by a disulfide bridge to the small subunit, which contains 17 residues. Its gene was cloned from a cDNA library. The nucleotide sequence showed that the same RNA messenger encodes both subunits, separated only by a pentapeptide, that is processed during maturation, (C) 1999 Federation of European Biochemical Societies.

引用

页码：447 / 450

页数：4

共 13 条

[1] Toxic peptides and genes encoding toxin gamma of the Brazilian scorpions Tityus bahiensis and Tityus stigmurus [J].

Becerril, B ;

Corona, M ;

Coronas, FIV ;

Zamudio, F ;

CAlderonAranda, ES ;

Fletcher, PL ;

Martin, BM ;

Possani, LD .

BIOCHEMICAL JOURNAL, 1996, 313 :753-760

[2]

BENISHIN CG, 1990, MOL PHARMACOL, V38, P164

[3]

Choumet V, 1996, ADV EXP MED BIOL, V391, P197

[4]

DENNIS EA, 1994, J BIOL CHEM, V269, P13057

[5] The growing phospholipase A(2) superfamily of signal transduction enzymes [J].

Dennis, EA .

TRENDS IN BIOCHEMICAL SCIENCES, 1997, 22 (01) :1-2

[6] SENSITIVE AND SPECIFIC PLATE TEST FOR QUANTITATION OF PHOSPHOLIPASES [J].

HABERMANN, E ;

HARDT, KL .

ANALYTICAL BIOCHEMISTRY, 1972, 50 (01) :163-+

[7] AMINO-ACID-SEQUENCE OF BETA-2-BUNGAROTOXIN FROM BUNGARUS-MULTICINCTUS VENOM - THE AMINO-ACID SUBSTITUTIONS IN THE B-CHAINS [J].

KONDO, K ;

TODA, H ;

NARITA, K ;

LEE, CY .

JOURNAL OF BIOCHEMISTRY, 1982, 91 (05) :1519-1530

[8] MYOTOXIC COMPONENTS OF SNAKE-VENOMS - THEIR BIOCHEMICAL AND BIOLOGICAL-ACTIVITIES [J].

MEBS, D ;

OWNBY, CL .

PHARMACOLOGY & THERAPEUTICS, 1990, 48 (02) :223-236

[9] BIOCHEMICAL-CHARACTERIZATION OF THE PHOSPHOLIPASE-A2 PURIFIED FROM THE VENOM OF THE MEXICAN BEADED LIZARD (HELODERMA-HORRIDUM HORRIDUM WIEGMANN) [J].

SOSA, BP ;

ALAGON, AC ;

MARTIN, BM ;

POSSANI, LD .

BIOCHEMISTRY, 1986, 25 (10) :2927-2933

[10] BETA-BUNGAROTOXIN, A PRE-SYNAPTIC TOXIN WITH ENZYMATIC-ACTIVITY [J].

STRONG, PN ;

GOERKE, J ;

OBERG, SG ;

KELLY, RB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (01) :178-182

← 1 2 →