Parvovirus B19 empty Capsids as antigen carriers for presentation of antigenic determinants of dengue 2 virus

被引：26

作者：

Amexis, Georgios ^{[1
]}

Young, Neal S. ^{[1
]}

机构：

[1] NHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 2006年 / 194卷 / 06期

关键词：

D O I：

10.1086/506361

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

For the production of dengue-vaccine candidates, empty capsids, or virus-like particles (VLPs), of parvovirus B19 that carry dengue 2-specific epitopes were employed as antigen carriers. Two epitopes ( comprising amino acids 352-368 and 386-397) of domain BIII of the envelope glycoprotein were chosen to produce recombinant B19 VLPs for immunization of BALB/c mice. Serum samples from immunized mice revealed that recombinant B19 VLPs elicited strong humoral immune responses. In summary, this B19 VLP-vaccine platform produced high (>= 2.0 x 105) anti-dengue 2 titers and robust ( <= 1: 120) 50%-plaque-reduction neutralization test (PRNT50) titers, which effectively neutralized live dengue 2 virus in PRNT50 assays.

引用

页码：790 / 794

页数：5

共 20 条

[1] Safety and immunogenicity of a recombinant parvovirus B19 vaccine formulated with MF59C.1 [J].