Bordetella pertussis adenylate cyclase toxin:: a versatile screening tool

被引：10

作者：

Dautin, N ^{[1
]}

Karimova, G ^{[1
]}

Ladant, D ^{[1
]}

机构：

[1] Inst Pasteur, Dept Struct Biol & Chem, CNRS URA 2185, F-75724 Paris 15, France

来源：

TOXICON | 2002年 / 40卷 / 10期

关键词：

bacteria; Bordetella pertussis; adenylate cyclase;

D O I：

10.1016/S0041-0101(02)00158-7

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The calmodulin-activated adenylate cyclase (AC) toxin is an essential virulence factor of Bordetella pertussis, the causative agent of whooping cough. This toxin has been exploited to devise screening techniques for investigating diverse biological processes. This mini-review describes several such applications. First, AC has been utilized as a selective reporter for protein translocation from bacteria to eukaryotic cells, in particular to study protein targeting by type III secretion machinery. More recently, AC has been used as a signal transducer in Escherichia coli to elaborate genetic screens for protein-protein interactions ("bacterial two-hybrid system") or site-specific proteolytic activities. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1383 / 1387

页数：5

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