Technique of the modulated chlorophyll fluorescence:: basic concepts, useful parameters, and some applications

被引：310

作者：

Rohácek, K

Barták, M

机构：

[1] Acad Sci Czech Republ, Inst Plant Mol Biol, Dept Photosynth, CZ-37005 Ceske Budejovice, Czech Republic

[2] Masaryk Univ, Dept Plant Physiol & Anat, CZ-61137 Brno, Czech Republic

来源：

PHOTOSYNTHETICA | 1999年 / 37卷 / 03期

关键词：

chemicals; circadien cycle; dark-adapted stale; fast kinetics; fluoranthene; light-adapted state; photosystem; 2; slow kinetics; temperature;

D O I：

10.1023/A:1007172424619

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The review summarizes basic information about slow and fast chlorophyll (Chl) a fluorescence induction kinetics (FIK) recorded using fluorimeters working on a principle of the pulse amplitude modulation (PAM) of a Chi fluorescence signal. It explains fundamental principles of the measuring technique, evaluates the terminology, symbols, and parameters used. Analysis of Chi FIK resulting in a set of Chl fluorescence parameters (FPs) provides qualitative and quantitative information about photosynthetic processes in chloroplasts. Using FPs, one can describe the functioning of the photosynthetic apparatus under different internal and external conditions. Brief comments on proper application of the fluorimetric method in photosynthesis research and some actual examples are also given.

引用

页码：339 / 363

页数：25

共 60 条

[1] The susceptibility of PS II of Lolium perenne to a sudden fall in air temperature -: response of plants grown in elevated CO2 and/or increased air temperature [J].