Identification of Cancer-Associated Fibroblasts in Circulating Blood from Patients with Metastatic Breast Cancer

被引：205

作者：

Ao, Zheng ^{[1
]}

Shah, Sanket H. ^{[1
]}

Machlin, Leah M. ^{[2
]}

Parajuli, Ritesh ^{[3
]}

Miller, Philip C. ^{[2
]}

Rawal, Siddarth ^{[4
]}

Williams, Anthony J. ^{[4
]}

Cote, Richard J. ^{[2
,4
]}

Lippman, Marc E. ^{[2
,3
]}

Datar, Ram H. ^{[2
,4
]}

El-Ashry, Dorraya ^{[2
,3
]}

机构：

[1] Univ Miami, Miller Sch Med, Sheila & David Fuente Grad Program Canc Biol, Miami, FL 33136 USA

[2] Sylvester Comprehens Canc Ctr, Miami, FL USA

[3] Univ Miami, Miller Sch Med, Dept Med, Div Hematol & Oncol, Miami, FL 33136 USA

[4] Univ Miami, Miller Sch Med, Dept Pathol, Miami, FL 33136 USA

来源：

CANCER RESEARCH | 2015年 / 75卷 / 22期

关键词：

TUMOR-CELLS; MACROPHAGES; SURVIVAL;

D O I：

10.1158/0008-5472.CAN-15-1633

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Metastasis is facilitated by cancer-associated fibroblasts (CAF) in the tumor microenvironment through mechanisms yet to be elucidated. In this study, we used a size-based microfilter technology developed by our group to examine whether circulating CAF identified by FAP and alpha-SMA coexpression (cCAF) could be distinguished in the peripheral blood of patients with metastatic breast cancer. In a pilot study of patients with breast cancer, we detected the presence of cCAFs in 30/34 (88%) patients with metastatic disease (MET group) and in 3/13 (23%) patients with localized breast cancer (LOC group) with long-term disease-free survival. No cCAFs as defined were detected in healthy donors. Further, both cCAF and circulating tumor cells (CTC) were significantly greater in the MET group compared with the LOC group. Thus, the presence of cCAF was associated with clinical metastasis, suggesting that cCAF may complement CTC as a clinically relevant biomarker in metastatic breast cancer. (C) 2015 AACR.

引用

页码：4681 / 4687

页数：7

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