Utilization of phytate phosphorus and calcium as influenced by microbial phytase, cholecalciferol, and the calcium:total phosphorus ratio in broiler diets

The present study was performed to evaluate the potential of microbial phytase and cholecalciferol (D-3) for improving the utilization of phytate P and Ca and the influence of the Ca:total (t) P ratio in a corn-soybean meal diet fed to broilers from hatch to 21 d of age. A 4 x 4 x 2 factorial arrangement of treatments was used: 1.1, 1.4, 1.7, and 2.0:1 Ca:tP ratio; 0, 300, 600, and 900 U of phytase/kg of diet; and 66 and 660 mu g of D-3/kg of diet. Another four treatments were included: the four Ca:tP ratios with 6,600 mu g of D-3 addition, but without phytase. Added phytase linearly increased (P < 0.001) BW gain, feed intake, toe ash content, and P and Ca retention; these measurements were negatively influenced by widening the dietary Ca:tP ratio, and synergetically improved by addition of D-3. Increasing the Ca:tP ratio decreased (P < 0.001) all measurements in the presence or absence of supplemental phytase and D-3 Dietary Ca:tP ratios between 1.1:1 to 1.4:1 appears critical to the efficient use of supplemental phytase and D-3 for improving the utilization of phytate P and Ca. The addition of D-3 in corn-soybean meal diets indicated a potential for improving the utilization of phytate P and Ca by increasing Ca and P retention by about 5 to 12% in birds, which led to an increase in toe ash content (P < 0.03). The enhanced phytate P utilization (P < 0.001) was also observed during assay of the phytase activity in the mixed diets with an addition of Dg and without added phytase in summary, the findings of this study suggested that phytase, D-3, and Ca:tP are important factors in degrading phytate and improving phytate P and Ca utilization in broilers.