Method for the simultaneous determination of Aconitum alkaloids and their hydrolysis products by gas chromatography mass spectrometry in human serum

被引：42

作者：

Ito, K ^{[1
]}

Ohyama, Y ^{[1
]}

Konishi, Y ^{[1
]}

Tanaka, S ^{[1
]}

Mizugaki, M ^{[1
]}

机构：

[1] TOHOKU UNIV HOSP,DEPT PHARMACEUT SCI,AOBA KU,SENDAI,MIYAGI 98077,JAPAN

来源：

PLANTA MEDICA | 1997年 / 63卷 / 01期

关键词：

Ranunculaceae; Aconitum alkaloids; GC-SIM; determination of Aconitum alkaloids; human serum;

D O I：

10.1055/s-2006-957609

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

This paper describes a newly developed method using gas chromatography-selected ion monitoring (GC-SIM) for the simultaneous determination of diester-diterpene type Aconitum alkaloids and their hydrolysis products in human serum. When the alkaloids were converted into their TMS derivatives, they produced a well defined peak on selected ion recording (SIR). Good linear response over the range of 100 pg to 7.5 ng was demonstrated for each alkaloid. When the alkaloids were spiked into human serum, good quantitative recovery was observed.

引用

页码：75 / 79

页数：5

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